| Fr., 23.10.2009 | SV Wilhelmshaven - SV Babelsberg 03 | 1:2 |
| Fr., 23.10.2009 | 1. FC Magdeburg - Hertha BSC Berlin II | 3:0 |
| Sa., 24.10.2009 | Tennis Borussia Berlin - VfL Wolfsburg II | 0:2 |
| Sa., 24.10.2009 | FC Oberneuland 1948 - VfB Lübeck | 0:2 |
| Sa., 24.10.2009 | FC Hansa Rostock II - Hallescher FC | 0:2 |
| Sa., 24.10.2009 | Türkiyemspor Berlin - Goslarer SC 08 | 3:2 |
| So., 25.10.2009 | ZFC Meuselwitz - Chemnitzer FC | 3:1 |
| So., 25.10.2009 | VFC Plauen - Hannover 96 II | 2:1 |
| So., 25.10.2009 | Hamburger SV II - FC St. Pauli II | 4:0 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | VfL Wolfsburg II | 12 | 8-3-1 | 25:4 | 21 | 27 |
| 2. | |  | SV Babelsberg 03 | 12 | 8-2-2 | 23:10 | 13 | 26 |
| 3. | |  | Hallescher FC | 12 | 7-4-1 | 24:9 | 15 | 25 |
| 4. |  |  | VfB Lübeck | 12 | 7-2-3 | 23:15 | 8 | 23 |
| 5. | |  | 1. FC Magdeburg | 12 | 6-4-2 | 27:14 | 13 | 22 |
| 6. |  |  | Chemnitzer FC | 12 | 6-3-3 | 23:14 | 9 | 21 |
| 7. | |  | Hertha BSC Berlin II | 12 | 6-1-5 | 21:19 | 2 | 19 |
| 8. | |  | Türkiyemspor Berlin | 12 | 6-1-5 | 18:19 | -1 | 19 |
| 9. | |  | Hamburger SV II | 12 | 4-5-3 | 16:15 | 1 | 17 |
| 10. | |  | Hannover 96 II | 12 | 4-2-6 | 16:15 | 1 | 14 |
| 11. | |  | ZFC Meuselwitz | 12 | 3-5-4 | 15:18 | -3 | 14 |
| 12. | |  | Tennis Borussia Berlin | 12 | 4-2-6 | 9:16 | -7 | 14 |
| 13. | |  | FC St. Pauli II | 12 | 3-4-5 | 11:20 | -9 | 13 |
| 14. | |  | VFC Plauen | 12 | 3-3-6 | 11:18 | -7 | 12 |
| 15. | |  | SV Wilhelmshaven | 12 | 2-5-5 | 13:20 | -7 | 11 |
| 16. | |  | FC Oberneuland 1948 | 12 | 3-1-8 | 12:27 | -15 | 10 |
| 17. | |  | FC Hansa Rostock II | 12 | 3-1-8 | 14:30 | -16 | 10 |
| 18. | |  | Goslarer SC 08 | 12 | 1-0-11 | 10:28 | -18 | 3 |