| Sa., 31.10.2009 | VfB Lübeck - SV Babelsberg 03 | 0:1 |
| Sa., 31.10.2009 | Hannover 96 II - SV Wilhelmshaven | 5:2 |
| Sa., 31.10.2009 | FC Oberneuland 1948 - Tennis Borussia Berlin | 0:0 |
| Sa., 31.10.2009 | Hertha BSC Berlin II - Hamburger SV II | 0:1 |
| Sa., 31.10.2009 | FC St. Pauli II - Türkiyemspor Berlin | 2:2 |
| Sa., 31.10.2009 | Goslarer SC 08 - FC Hansa Rostock II | 1:1 |
| So., 01.11.2009 | VfL Wolfsburg II - ZFC Meuselwitz | 0:0 |
| So., 01.11.2009 | Chemnitzer FC - 1. FC Magdeburg | 1:1 |
| So., 01.11.2009 | Hallescher FC - VFC Plauen | 0:0 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | SV Babelsberg 03 | 13 | 9-2-2 | 24:10 | 14 | 29 |
| 2. |  |  | VfL Wolfsburg II | 13 | 8-4-1 | 25:4 | 21 | 28 |
| 3. |  |  | Hallescher FC | 13 | 7-5-1 | 24:9 | 15 | 26 |
| 4. | |  | 1. FC Magdeburg | 13 | 6-5-2 | 28:15 | 13 | 23 |
| 5. | |  | VfB Lübeck | 13 | 7-2-4 | 23:16 | 7 | 23 |
| 6. | |  | Chemnitzer FC | 13 | 6-4-3 | 24:15 | 9 | 22 |
| 7. | |  | Hamburger SV II | 13 | 5-5-3 | 17:15 | 2 | 20 |
| 8. | |  | Türkiyemspor Berlin | 13 | 6-2-5 | 20:21 | -1 | 20 |
| 9. | |  | Hertha BSC Berlin II | 13 | 6-1-6 | 21:20 | 1 | 19 |
| 10. | |  | Hannover 96 II | 13 | 5-2-6 | 21:17 | 4 | 17 |
| 11. | |  | ZFC Meuselwitz | 13 | 3-6-4 | 15:18 | -3 | 15 |
| 12. | |  | Tennis Borussia Berlin | 13 | 4-3-6 | 9:16 | -7 | 15 |
| 13. | |  | FC St. Pauli II | 13 | 3-5-5 | 13:22 | -9 | 14 |
| 14. | |  | VFC Plauen | 13 | 3-4-6 | 11:18 | -7 | 13 |
| 15. | |  | SV Wilhelmshaven | 13 | 2-5-6 | 15:25 | -10 | 11 |
| 16. | |  | FC Oberneuland 1948 | 13 | 3-2-8 | 12:27 | -15 | 11 |
| 17. | |  | FC Hansa Rostock II | 13 | 3-2-8 | 15:31 | -16 | 11 |
| 18. | |  | Goslarer SC 08 | 13 | 1-1-11 | 11:29 | -18 | 4 |