| Fr., 25.02.2000 | Kickers Offenbach - Chemnitzer FC | 2:0 |
| Fr., 25.02.2000 | VfL Bochum - FC St. Pauli | 2:0 |
| Fr., 25.02.2000 | Tennis Borussia Berlin - Alemannia Aachen | 1:2 |
| Sa., 26.02.2000 | FSV Mainz 05 - FC Energie Cottbus | 1:0 |
| So., 27.02.2000 | Stuttgarter Kickers - 1. FC Köln | 1:2 |
| So., 27.02.2000 | SV Waldhof Mannheim - Hannover 96 | 2:2 |
| So., 27.02.2000 | SC Fortuna Köln - 1. FC Nürnberg | 1:1 |
| So., 27.02.2000 | SpVgg Greuther Fürth - Rot-Weiß Oberhausen | 0:1 |
| Mo., 28.02.2000 | Borussia Mönchengladbach - Karlsruher SC | 4:1 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | 1. FC Köln | 20 | 13-5-2 | 43:16 | 27 | 44 |
| 2. | |  | VfL Bochum | 20 | 11-3-6 | 35:27 | 8 | 36 |
| 3. | |  | FC Energie Cottbus | 20 | 10-1-9 | 37:25 | 12 | 31 |
| 4. |  |  | Borussia Mönchengladbach | 20 | 8-6-6 | 31:24 | 7 | 30 |
| 5. |  |  | Tennis Borussia Berlin | 20 | 7-9-4 | 27:22 | 5 | 30 |
| 6. | |  | Alemannia Aachen | 20 | 8-6-6 | 30:34 | -4 | 30 |
| 7. | |  | 1. FC Nürnberg | 20 | 8-5-7 | 30:35 | -5 | 29 |
| 8. | |  | SV Waldhof Mannheim | 20 | 7-7-6 | 29:28 | 1 | 28 |
| 9. | |  | FSV Mainz 05 | 20 | 6-9-5 | 23:23 | 0 | 27 |
| 10. | |  | Rot-Weiß Oberhausen | 20 | 6-8-6 | 18:20 | -2 | 26 |
| 11. | |  | Chemnitzer FC | 20 | 6-7-7 | 27:25 | 2 | 25 |
| 12. | |  | SpVgg Greuther Fürth | 20 | 5-10-5 | 14:18 | -4 | 25 |
| 13. | |  | Hannover 96 | 20 | 6-5-9 | 29:30 | -1 | 23 |
| 14. | |  | FC St. Pauli | 20 | 5-8-7 | 21:26 | -5 | 23 |
| 15. | |  | Stuttgarter Kickers | 20 | 5-5-10 | 29:38 | -9 | 20 |
| 16. | |  | Kickers Offenbach | 20 | 4-8-8 | 20:31 | -11 | 20 |
| 17. | |  | SC Fortuna Köln | 20 | 4-8-8 | 18:29 | -11 | 20 |
| 18. | |  | Karlsruher SC | 20 | 2-8-10 | 23:33 | -10 | 14 |