| Mi., 22.03.2000 | 1. FC Nürnberg - Hannover 96 | 5:1 |
| Fr., 24.03.2000 | Kickers Offenbach - FC St. Pauli | 0:1 |
| Fr., 24.03.2000 | Tennis Borussia Berlin - SpVgg Greuther Fürth | 3:1 |
| Sa., 25.03.2000 | VfL Bochum - Stuttgarter Kickers | 2:1 |
| So., 26.03.2000 | Borussia Mönchengladbach - Rot-Weiß Oberhausen | 4:2 |
| So., 26.03.2000 | SV Waldhof Mannheim - 1. FC Köln | 2:1 |
| So., 26.03.2000 | SC Fortuna Köln - Alemannia Aachen | 0:0 |
| So., 26.03.2000 | FSV Mainz 05 - Karlsruher SC | 2:2 |
| Mo., 27.03.2000 | Chemnitzer FC - FC Energie Cottbus | 1:2 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | 1. FC Köln | 24 | 14-6-4 | 48:21 | 27 | 48 |
| 2. | |  | VfL Bochum | 24 | 13-4-7 | 40:33 | 7 | 43 |
| 3. | |  | FC Energie Cottbus | 24 | 13-2-9 | 45:29 | 16 | 41 |
| 4. | |  | Borussia Mönchengladbach | 24 | 10-8-6 | 39:29 | 10 | 38 |
| 5. |  |  | Tennis Borussia Berlin | 24 | 9-10-5 | 33:27 | 6 | 37 |
| 6. | |  | 1. FC Nürnberg | 24 | 10-7-7 | 39:38 | 1 | 37 |
| 7. |  |  | Alemannia Aachen | 24 | 9-9-6 | 34:37 | -3 | 36 |
| 8. | |  | SV Waldhof Mannheim | 24 | 8-10-6 | 34:32 | 2 | 34 |
| 9. | |  | FSV Mainz 05 | 24 | 7-10-7 | 31:29 | 2 | 31 |
| 10. | |  | Rot-Weiß Oberhausen | 24 | 7-9-8 | 25:27 | -2 | 30 |
| 11. | |  | Chemnitzer FC | 24 | 7-8-9 | 31:34 | -3 | 29 |
| 12. | |  | SpVgg Greuther Fürth | 24 | 6-11-7 | 24:28 | -4 | 29 |
| 13. | |  | Hannover 96 | 24 | 7-7-10 | 34:38 | -4 | 28 |
| 14. | |  | FC St. Pauli | 24 | 6-10-8 | 25:30 | -5 | 28 |
| 15. | |  | Kickers Offenbach | 24 | 5-9-10 | 23:35 | -12 | 24 |
| 16. | |  | SC Fortuna Köln | 24 | 4-10-10 | 21:34 | -13 | 22 |
| 17. | |  | Stuttgarter Kickers | 24 | 5-6-13 | 33:48 | -15 | 21 |
| 18. | |  | Karlsruher SC | 24 | 3-10-11 | 28:38 | -10 | 19 |