| Fr., 14.04.2000 | Alemannia Aachen - Chemnitzer FC | 0:1 |
| Fr., 14.04.2000 | Stuttgarter Kickers - SV Waldhof Mannheim | 3:0 |
| Fr., 14.04.2000 | FC St. Pauli - 1. FC Nürnberg | 0:0 |
| Sa., 15.04.2000 | FC Energie Cottbus - Tennis Borussia Berlin | 2:0 |
| So., 16.04.2000 | 1. FC Köln - FSV Mainz 05 | 1:0 |
| So., 16.04.2000 | Rot-Weiß Oberhausen - SC Fortuna Köln | 4:0 |
| So., 16.04.2000 | Karlsruher SC - Hannover 96 | 1:3 |
| So., 16.04.2000 | SpVgg Greuther Fürth - Kickers Offenbach | 1:1 |
| Mo., 17.04.2000 | VfL Bochum - Borussia Mönchengladbach | 2:2 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | 1. FC Köln | 27 | 15-7-5 | 51:26 | 25 | 52 |
| 2. | |  | VfL Bochum | 27 | 14-6-7 | 47:36 | 11 | 48 |
| 3. |  |  | FC Energie Cottbus | 27 | 14-3-10 | 50:33 | 17 | 45 |
| 4. |  |  | 1. FC Nürnberg | 27 | 12-8-7 | 43:39 | 4 | 44 |
| 5. | |  | Borussia Mönchengladbach | 27 | 10-11-6 | 43:33 | 10 | 41 |
| 6. | |  | Alemannia Aachen | 27 | 10-9-8 | 36:41 | -5 | 39 |
| 7. | |  | Hannover 96 | 27 | 10-7-10 | 42:41 | 1 | 37 |
| 8. | |  | Tennis Borussia Berlin | 27 | 9-10-8 | 33:35 | -2 | 37 |
| 9. | |  | SV Waldhof Mannheim | 27 | 8-12-7 | 38:39 | -1 | 36 |
| 10. | |  | Chemnitzer FC | 27 | 9-8-10 | 35:37 | -2 | 35 |
| 11. | |  | Rot-Weiß Oberhausen | 27 | 8-10-9 | 31:30 | 1 | 34 |
| 12. | |  | FSV Mainz 05 | 27 | 7-11-9 | 32:32 | 0 | 32 |
| 13. | |  | SpVgg Greuther Fürth | 27 | 6-14-7 | 29:33 | -4 | 32 |
| 14. | |  | FC St. Pauli | 27 | 6-13-8 | 26:31 | -5 | 31 |
| 15. | |  | Kickers Offenbach | 27 | 6-11-10 | 27:38 | -11 | 29 |
| 16. | |  | Stuttgarter Kickers | 27 | 7-7-13 | 39:49 | -10 | 28 |
| 17. | |  | SC Fortuna Köln | 27 | 5-11-11 | 25:39 | -14 | 26 |
| 18. | |  | Karlsruher SC | 27 | 3-10-14 | 30:45 | -15 | 19 |