| Fr., 06.03.2009 | SV Babelsberg 03 - FC Energie Cottbus II | 1:1 |
| Fr., 06.03.2009 | VfL Wolfsburg II - FC Sachsen Leipzig | 0:1 |
| Sa., 07.03.2009 | Chemnitzer FC - FC Oberneuland 1948 | 0:0 |
| Sa., 07.03.2009 | FC Hansa Rostock II - VFC Plauen | 2:0 |
| Sa., 07.03.2009 | Holstein Kiel - Altonaer FC 93 | 4:0 |
| Sa., 07.03.2009 | Hamburger SV II - SV Wilhelmshaven | 2:0 |
| Sa., 07.03.2009 | Hertha BSC Berlin II - VfB Lübeck | 0:0 |
| Sa., 07.03.2009 | Hannover 96 II - Türkiyemspor Berlin | 1:2 |
| So., 08.03.2009 | Hallescher FC - 1. FC Magdeburg | 1:0 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | Holstein Kiel | 21 | 14-5-2 | 37:16 | 21 | 47 |
| 2. | |  | Hallescher FC | 21 | 12-9-0 | 24:8 | 16 | 45 |
| 3. | |  | 1. FC Magdeburg | 21 | 12-5-4 | 31:16 | 15 | 41 |
| 4. | |  | SV Babelsberg 03 | 21 | 10-10-1 | 26:10 | 16 | 40 |
| 5. |  |  | Hannover 96 II | 21 | 9-3-9 | 30:26 | 4 | 30 |
| 6. |  |  | SV Wilhelmshaven | 21 | 8-6-7 | 27:27 | 0 | 30 |
| 7. | |  | FC Hansa Rostock II | 19 | 9-2-8 | 33:28 | 5 | 29 |
| 8. | |  | VfL Wolfsburg II | 19 | 7-4-8 | 23:18 | 5 | 25 |
| 9. | |  | VfB Lübeck | 21 | 5-10-6 | 20:20 | 0 | 25 |
| 10. | |  | Chemnitzer FC | 19 | 6-6-7 | 27:23 | 4 | 24 |
| 11. | |  | VFC Plauen | 19 | 6-6-7 | 25:27 | -2 | 24 |
| 12. | |  | FC Oberneuland 1948 | 19 | 6-5-8 | 17:19 | -2 | 23 |
| 13. | |  | Türkiyemspor Berlin | 19 | 7-2-10 | 19:26 | -7 | 23 |
| 14. | |  | Altonaer FC 93 | 20 | 6-4-10 | 24:37 | -13 | 22 |
| 15. | |  | Hertha BSC Berlin II | 20 | 5-6-9 | 19:33 | -14 | 21 |
| 16. | |  | Hamburger SV II | 20 | 5-5-10 | 21:26 | -5 | 20 |
| 17. | |  | FC Sachsen Leipzig | 20 | 3-7-10 | 10:27 | -17 | 16 |
| 18. | |  | FC Energie Cottbus II | 21 | 1-5-15 | 13:39 | -26 | 8 |