| Sa., 14.03.2009 | Chemnitzer FC - FC Hansa Rostock II | 2:0 |
| Sa., 14.03.2009 | FC Energie Cottbus II - Hallescher FC | 0:0 |
| Sa., 14.03.2009 | Altonaer FC 93 - SV Babelsberg 03 | 1:2 |
| Sa., 14.03.2009 | FC Oberneuland 1948 - Hannover 96 II | 0:2 |
| Sa., 14.03.2009 | VfB Lübeck - VfL Wolfsburg II | 1:1 |
| So., 15.03.2009 | 1. FC Magdeburg - Hamburger SV II | 2:1 |
| So., 15.03.2009 | Türkiyemspor Berlin - Holstein Kiel | 0:1 |
| So., 15.03.2009 | SV Wilhelmshaven - Hertha BSC Berlin II | 0:2 |
| Mi., 29.04.2009 | FC Sachsen Leipzig - VFC Plauen | 0:3 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | Holstein Kiel | 22 | 15-5-2 | 38:16 | 22 | 50 |
| 2. | |  | Hallescher FC | 22 | 12-10-0 | 24:8 | 16 | 46 |
| 3. | |  | 1. FC Magdeburg | 22 | 13-5-4 | 33:17 | 16 | 44 |
| 4. | |  | SV Babelsberg 03 | 22 | 11-10-1 | 28:11 | 17 | 43 |
| 5. | |  | Hannover 96 II | 22 | 10-3-9 | 32:26 | 6 | 33 |
| 6. | |  | SV Wilhelmshaven | 22 | 8-6-8 | 27:29 | -2 | 30 |
| 7. | |  | FC Hansa Rostock II | 20 | 9-2-9 | 33:30 | 3 | 29 |
| 8. |  |  | Chemnitzer FC | 20 | 7-6-7 | 29:23 | 6 | 27 |
| 9. |  |  | VfL Wolfsburg II | 20 | 7-5-8 | 24:19 | 5 | 26 |
| 10. | |  | VfB Lübeck | 22 | 5-11-6 | 21:21 | 0 | 26 |
| 11. | |  | VFC Plauen | 19 | 6-6-7 | 25:27 | -2 | 24 |
| 12. | |  | FC Oberneuland 1948 | 21 | 6-6-9 | 19:23 | -4 | 24 |
| 13. | |  | Türkiyemspor Berlin | 21 | 7-3-11 | 21:29 | -8 | 24 |
| 14. | |  | Hertha BSC Berlin II | 21 | 6-6-9 | 21:33 | -12 | 24 |
| 15. | |  | Altonaer FC 93 | 21 | 6-4-11 | 25:39 | -14 | 22 |
| 16. | |  | Hamburger SV II | 21 | 5-5-11 | 22:28 | -6 | 20 |
| 17. | |  | FC Sachsen Leipzig | 20 | 3-7-10 | 10:27 | -17 | 16 |
| 18. | |  | FC Energie Cottbus II | 22 | 1-6-15 | 13:39 | -26 | 9 |