| Sa., 28.03.2009 | Hannover 96 II - FC Hansa Rostock II | 1:1 |
| Sa., 28.03.2009 | Altonaer FC 93 - Hamburger SV II | 1:3 |
| Sa., 28.03.2009 | FC Oberneuland 1948 - SV Babelsberg 03 | 2:0 |
| Sa., 28.03.2009 | FC Energie Cottbus II - Hertha BSC Berlin II | 1:0 |
| So., 29.03.2009 | Chemnitzer FC - Holstein Kiel | 0:1 |
| So., 29.03.2009 | Türkiyemspor Berlin - Hallescher FC | 2:2 |
| So., 29.03.2009 | 1. FC Magdeburg - VfL Wolfsburg II | 0:3 |
| Di., 31.03.2009 | VfB Lübeck - FC Sachsen Leipzig | 1:1 |
| Mi., 22.04.2009 | SV Wilhelmshaven - VFC Plauen | 2:2 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | Holstein Kiel | 24 | 16-6-2 | 40:17 | 23 | 54 |
| 2. | |  | Hallescher FC | 24 | 13-11-0 | 28:10 | 18 | 50 |
| 3. | |  | SV Babelsberg 03 | 24 | 12-10-2 | 29:13 | 16 | 46 |
| 4. | |  | 1. FC Magdeburg | 24 | 13-5-6 | 33:21 | 12 | 44 |
| 5. |  |  | VfL Wolfsburg II | 23 | 10-5-8 | 37:22 | 15 | 35 |
| 6. |  |  | Hannover 96 II | 24 | 10-5-9 | 34:28 | 6 | 35 |
| 7. | |  | FC Hansa Rostock II | 23 | 10-3-10 | 37:36 | 1 | 33 |
| 8. | |  | FC Oberneuland 1948 | 24 | 8-7-9 | 25:24 | 1 | 31 |
| 9. | |  | SV Wilhelmshaven | 23 | 8-6-9 | 29:34 | -5 | 30 |
| 10. | |  | Chemnitzer FC | 23 | 7-8-8 | 31:26 | 5 | 29 |
| 11. | |  | VfB Lübeck | 24 | 5-13-6 | 25:25 | 0 | 28 |
| 12. | |  | Hertha BSC Berlin II | 23 | 7-6-10 | 22:34 | -12 | 27 |
| 13. | |  | VFC Plauen | 21 | 6-7-8 | 29:35 | -6 | 25 |
| 14. | |  | Türkiyemspor Berlin | 23 | 7-4-12 | 23:32 | -9 | 25 |
| 15. | |  | Hamburger SV II | 23 | 6-5-12 | 25:30 | -5 | 23 |
| 16. | |  | Altonaer FC 93 | 24 | 6-5-13 | 27:45 | -18 | 23 |
| 17. | |  | FC Sachsen Leipzig | 22 | 3-8-11 | 13:31 | -18 | 17 |
| 18. | |  | FC Energie Cottbus II | 24 | 3-6-15 | 15:39 | -24 | 15 |