| Sa., 04.04.2009 | FC Sachsen Leipzig - SV Wilhelmshaven | 3:1 |
| Sa., 04.04.2009 | Hallescher FC - FC Oberneuland 1948 | 1:0 |
| So., 05.04.2009 | Hertha BSC Berlin II - Altonaer FC 93 | 4:3 |
| So., 05.04.2009 | VfL Wolfsburg II - FC Energie Cottbus II | 0:1 |
| So., 05.04.2009 | Hamburger SV II - Türkiyemspor Berlin | 0:0 |
| So., 05.04.2009 | Holstein Kiel - Hannover 96 II | 2:0 |
| Sa., 11.04.2009 | SV Babelsberg 03 - Chemnitzer FC | 3:1 |
| Sa., 11.04.2009 | FC Hansa Rostock II - VfB Lübeck | 1:1 |
| So., 12.04.2009 | VFC Plauen - 1. FC Magdeburg | 3:4 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | Holstein Kiel | 25 | 17-6-2 | 42:17 | 25 | 57 |
| 2. | |  | Hallescher FC | 25 | 14-11-0 | 29:10 | 19 | 53 |
| 3. | |  | SV Babelsberg 03 | 25 | 13-10-2 | 32:14 | 18 | 49 |
| 4. | |  | 1. FC Magdeburg | 25 | 14-5-6 | 37:24 | 13 | 47 |
| 5. | |  | VfL Wolfsburg II | 24 | 10-5-9 | 37:23 | 14 | 35 |
| 6. | |  | Hannover 96 II | 25 | 10-5-10 | 34:30 | 4 | 35 |
| 7. | |  | FC Hansa Rostock II | 24 | 10-4-10 | 38:37 | 1 | 34 |
| 8. |  |  | Hertha BSC Berlin II | 25 | 9-6-10 | 27:37 | -10 | 33 |
| 9. |  |  | FC Oberneuland 1948 | 25 | 8-7-10 | 25:25 | 0 | 31 |
| 10. | |  | SV Wilhelmshaven | 24 | 8-6-10 | 30:37 | -7 | 30 |
| 11. | |  | Chemnitzer FC | 24 | 7-8-9 | 32:29 | 3 | 29 |
| 12. | |  | VfB Lübeck | 25 | 5-14-6 | 26:26 | 0 | 29 |
| 13. | |  | Hamburger SV II | 25 | 7-6-12 | 29:30 | -1 | 27 |
| 14. | |  | Türkiyemspor Berlin | 24 | 7-5-12 | 23:32 | -9 | 26 |
| 15. | |  | VFC Plauen | 23 | 6-7-10 | 32:40 | -8 | 25 |
| 16. | |  | Altonaer FC 93 | 25 | 6-5-14 | 30:49 | -19 | 23 |
| 17. | |  | FC Sachsen Leipzig | 24 | 4-8-12 | 16:36 | -20 | 20 |
| 18. | |  | FC Energie Cottbus II | 25 | 4-6-15 | 16:39 | -23 | 18 |