| Fr., 17.04.2009 | 1. FC Magdeburg - FC Sachsen Leipzig | 0:0 |
| Sa., 18.04.2009 | Holstein Kiel - FC Hansa Rostock II | 1:1 |
| Sa., 18.04.2009 | Hannover 96 II - SV Babelsberg 03 | 0:1 |
| Sa., 18.04.2009 | SV Wilhelmshaven - VfB Lübeck | 2:1 |
| Sa., 18.04.2009 | Altonaer FC 93 - VfL Wolfsburg II | 0:1 |
| Sa., 18.04.2009 | Türkiyemspor Berlin - Hertha BSC Berlin II | 0:1 |
| So., 19.04.2009 | Chemnitzer FC - Hallescher FC | 1:3 |
| So., 19.04.2009 | FC Energie Cottbus II - VFC Plauen | 1:1 |
| So., 19.04.2009 | FC Oberneuland 1948 - Hamburger SV II | 0:3 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | Holstein Kiel | 26 | 17-7-2 | 43:18 | 25 | 58 |
| 2. | |  | Hallescher FC | 26 | 15-11-0 | 32:11 | 21 | 56 |
| 3. | |  | SV Babelsberg 03 | 26 | 14-10-2 | 33:14 | 19 | 52 |
| 4. | |  | 1. FC Magdeburg | 26 | 14-6-6 | 37:24 | 13 | 48 |
| 5. | |  | VfL Wolfsburg II | 26 | 11-6-9 | 40:25 | 15 | 39 |
| 6. |  |  | FC Hansa Rostock II | 26 | 10-6-10 | 41:40 | 1 | 36 |
| 7. | |  | Hertha BSC Berlin II | 26 | 10-6-10 | 28:37 | -9 | 36 |
| 8. |  |  | Hannover 96 II | 26 | 10-5-11 | 34:31 | 3 | 35 |
| 9. | |  | SV Wilhelmshaven | 26 | 9-7-10 | 34:40 | -6 | 34 |
| 10. | |  | Chemnitzer FC | 26 | 8-8-10 | 36:32 | 4 | 32 |
| 11. | |  | FC Oberneuland 1948 | 26 | 8-7-11 | 25:28 | -3 | 31 |
| 12. | |  | Hamburger SV II | 26 | 8-6-12 | 32:30 | 2 | 30 |
| 13. | |  | VfB Lübeck | 26 | 5-14-7 | 27:28 | -1 | 29 |
| 14. | |  | VFC Plauen | 25 | 6-9-10 | 35:43 | -8 | 27 |
| 15. | |  | Türkiyemspor Berlin | 26 | 7-5-14 | 23:36 | -13 | 26 |
| 16. | |  | Altonaer FC 93 | 26 | 6-5-15 | 30:50 | -20 | 23 |
| 17. | |  | FC Sachsen Leipzig | 25 | 4-9-12 | 16:36 | -20 | 21 |
| 18. | |  | FC Energie Cottbus II | 26 | 4-7-15 | 17:40 | -23 | 19 |