| Fr., 01.05.2009 | SV Babelsberg 03 - FC Hansa Rostock II | 2:0 |
| Sa., 02.05.2009 | Altonaer FC 93 - FC Sachsen Leipzig | 2:1 |
| Sa., 02.05.2009 | Hannover 96 II - Hamburger SV II | 1:1 |
| Sa., 02.05.2009 | FC Energie Cottbus II - VfB Lübeck | 1:2 |
| So., 03.05.2009 | Chemnitzer FC - Hertha BSC Berlin II | 3:3 |
| So., 03.05.2009 | Holstein Kiel - Hallescher FC | 1:2 |
| So., 03.05.2009 | 1. FC Magdeburg - SV Wilhelmshaven | 1:0 |
| So., 03.05.2009 | FC Oberneuland 1948 - VfL Wolfsburg II | 2:3 |
| Mo., 04.05.2009 | Türkiyemspor Berlin - VFC Plauen | 2:2 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | Holstein Kiel | 28 | 18-7-3 | 46:20 | 26 | 61 |
| 2. | |  | Hallescher FC | 28 | 16-11-1 | 34:14 | 20 | 59 |
| 3. | |  | SV Babelsberg 03 | 28 | 15-10-3 | 35:16 | 19 | 55 |
| 4. | |  | 1. FC Magdeburg | 28 | 15-6-7 | 38:25 | 13 | 51 |
| 5. | |  | VfL Wolfsburg II | 28 | 13-6-9 | 46:28 | 18 | 45 |
| 6. | |  | Hertha BSC Berlin II | 28 | 11-7-10 | 34:42 | -8 | 40 |
| 7. | |  | Hannover 96 II | 28 | 11-6-11 | 37:32 | 5 | 39 |
| 8. | |  | SV Wilhelmshaven | 28 | 10-7-11 | 39:43 | -4 | 37 |
| 9. | |  | FC Hansa Rostock II | 28 | 10-6-12 | 43:47 | -4 | 36 |
| 10. |  |  | VfB Lübeck | 28 | 7-14-7 | 30:29 | 1 | 35 |
| 11. |  |  | Chemnitzer FC | 28 | 8-10-10 | 41:37 | 4 | 34 |
| 12. | |  | VFC Plauen | 28 | 8-10-10 | 44:46 | -2 | 34 |
| 13. | |  | Hamburger SV II | 28 | 8-8-12 | 35:33 | 2 | 32 |
| 14. | |  | FC Oberneuland 1948 | 28 | 8-7-13 | 29:34 | -5 | 31 |
| 15. | |  | Türkiyemspor Berlin | 28 | 7-6-15 | 26:41 | -15 | 27 |
| 16. | |  | Altonaer FC 93 | 28 | 7-5-16 | 33:55 | -22 | 26 |
| 17. | |  | FC Sachsen Leipzig | 28 | 5-9-14 | 20:42 | -22 | 24 |
| 18. | |  | FC Energie Cottbus II | 28 | 4-7-17 | 19:45 | -26 | 19 |