| Mi., 13.05.2009 | Chemnitzer FC - VFC Plauen | 3:0 |
| Mi., 13.05.2009 | Hallescher FC - FC Hansa Rostock II | 2:1 |
| Mi., 13.05.2009 | SV Babelsberg 03 - Hamburger SV II | 6:1 |
| Mi., 13.05.2009 | Altonaer FC 93 - SV Wilhelmshaven | 2:0 |
| Mi., 13.05.2009 | Türkiyemspor Berlin - VfB Lübeck | 3:0 |
| Mi., 13.05.2009 | FC Oberneuland 1948 - FC Sachsen Leipzig | 2:0 |
| Mi., 13.05.2009 | Hannover 96 II - VfL Wolfsburg II | 1:2 |
| Mi., 13.05.2009 | Holstein Kiel - Hertha BSC Berlin II | 1:1 |
| Sa., 16.05.2009 | FC Energie Cottbus II - 1. FC Magdeburg | 0:2 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | Holstein Kiel | 30 | 19-8-3 | 49:21 | 28 | 65 |
| 2. | |  | Hallescher FC | 30 | 18-11-1 | 38:16 | 22 | 65 |
| 3. | |  | SV Babelsberg 03 | 30 | 16-10-4 | 42:19 | 23 | 58 |
| 4. |  |  | VfL Wolfsburg II | 30 | 15-6-9 | 51:30 | 21 | 51 |
| 5. |  |  | 1. FC Magdeburg | 29 | 15-6-8 | 40:29 | 11 | 51 |
| 6. | |  | Hannover 96 II | 30 | 12-6-12 | 41:34 | 7 | 42 |
| 7. | |  | Hertha BSC Berlin II | 30 | 11-8-11 | 35:46 | -11 | 41 |
| 8. | |  | SV Wilhelmshaven | 30 | 11-7-12 | 40:45 | -5 | 40 |
| 9. | |  | FC Hansa Rostock II | 30 | 11-6-13 | 48:51 | -3 | 39 |
| 10. | |  | VfB Lübeck | 30 | 8-14-8 | 32:32 | 0 | 38 |
| 11. | |  | Chemnitzer FC | 30 | 9-10-11 | 45:40 | 5 | 37 |
| 12. | |  | FC Oberneuland 1948 | 30 | 10-7-13 | 32:34 | -2 | 37 |
| 13. | |  | VFC Plauen | 30 | 8-10-12 | 44:50 | -6 | 34 |
| 14. | |  | Türkiyemspor Berlin | 30 | 9-6-15 | 31:41 | -10 | 33 |
| 15. | |  | Hamburger SV II | 30 | 8-8-14 | 36:41 | -5 | 32 |
| 16. | |  | Altonaer FC 93 | 30 | 8-5-17 | 35:57 | -22 | 29 |
| 17. | |  | FC Sachsen Leipzig | 30 | 5-9-16 | 20:46 | -26 | 24 |
| 18. | |  | FC Energie Cottbus II | 29 | 4-7-18 | 19:46 | -27 | 19 |