| Fr., 22.05.2009 | Türkiyemspor Berlin - 1. FC Magdeburg | 2:0 |
| Sa., 23.05.2009 | Altonaer FC 93 - FC Energie Cottbus II | 2:2 |
| Sa., 23.05.2009 | SV Babelsberg 03 - VfL Wolfsburg II | 0:0 |
| So., 24.05.2009 | Chemnitzer FC - VfB Lübeck | 1:1 |
| So., 24.05.2009 | Hannover 96 II - FC Sachsen Leipzig | 2:1 |
| So., 24.05.2009 | Holstein Kiel - VFC Plauen | 0:0 |
| So., 24.05.2009 | Hallescher FC - Hertha BSC Berlin II | 2:2 |
| So., 24.05.2009 | Hamburger SV II - FC Hansa Rostock II | 3:0 |
| So., 24.05.2009 | FC Oberneuland 1948 - SV Wilhelmshaven | 3:2 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | Holstein Kiel | 32 | 19-10-3 | 50:22 | 28 | 67 |
| 2. | |  | Hallescher FC | 32 | 18-13-1 | 41:19 | 22 | 67 |
| 3. | |  | SV Babelsberg 03 | 32 | 16-12-4 | 44:21 | 23 | 60 |
| 4. | |  | 1. FC Magdeburg | 32 | 17-6-9 | 46:32 | 14 | 57 |
| 5. | |  | VfL Wolfsburg II | 32 | 15-8-9 | 52:31 | 21 | 53 |
| 6. | |  | Hannover 96 II | 32 | 13-7-12 | 46:38 | 8 | 46 |
| 7. | |  | SV Wilhelmshaven | 32 | 12-7-13 | 44:49 | -5 | 43 |
| 8. | |  | Hertha BSC Berlin II | 32 | 11-10-11 | 39:50 | -11 | 43 |
| 9. | |  | VfB Lübeck | 32 | 9-15-8 | 35:34 | 1 | 42 |
| 10. | |  | Chemnitzer FC | 32 | 10-11-11 | 48:41 | 7 | 41 |
| 11. |  |  | FC Oberneuland 1948 | 32 | 11-7-14 | 36:38 | -2 | 40 |
| 12. |  |  | FC Hansa Rostock II | 32 | 11-6-15 | 48:56 | -8 | 39 |
| 13. | |  | Hamburger SV II | 32 | 9-9-14 | 40:42 | -2 | 36 |
| 14. | |  | VFC Plauen | 32 | 8-12-12 | 47:53 | -6 | 36 |
| 15. | |  | Türkiyemspor Berlin | 32 | 10-6-16 | 34:43 | -9 | 36 |
| 16. | |  | Altonaer FC 93 | 32 | 8-6-18 | 38:63 | -25 | 30 |
| 17. | |  | FC Sachsen Leipzig | 32 | 5-9-18 | 21:50 | -29 | 24 |
| 18. | |  | FC Energie Cottbus II | 32 | 5-8-19 | 23:50 | -27 | 23 |