| Sa., 30.05.2009 | SV Wilhelmshaven - Chemnitzer FC | 0:1 |
| Sa., 30.05.2009 | Altonaer FC 93 - FC Hansa Rostock II | 2:2 |
| Sa., 30.05.2009 | Hertha BSC Berlin II - Hamburger SV II | 0:2 |
| Sa., 30.05.2009 | VfL Wolfsburg II - Hallescher FC | 0:2 |
| Sa., 30.05.2009 | VFC Plauen - SV Babelsberg 03 | 4:1 |
| Sa., 30.05.2009 | FC Sachsen Leipzig - Holstein Kiel | 0:3 |
| Sa., 30.05.2009 | VfB Lübeck - Hannover 96 II | 3:0 |
| Sa., 30.05.2009 | 1. FC Magdeburg - FC Oberneuland 1948 | 3:3 |
| Sa., 30.05.2009 | FC Energie Cottbus II - Türkiyemspor Berlin | 0:1 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | Holstein Kiel | 33 | 20-10-3 | 53:22 | 31 | 70 |
| 2. | |  | Hallescher FC | 33 | 19-13-1 | 43:19 | 24 | 70 |
| 3. | |  | SV Babelsberg 03 | 33 | 16-12-5 | 45:25 | 20 | 60 |
| 4. | |  | 1. FC Magdeburg | 33 | 17-7-9 | 49:35 | 14 | 58 |
| 5. | |  | VfL Wolfsburg II | 33 | 15-8-10 | 52:33 | 19 | 53 |
| 6. | |  | Hannover 96 II | 33 | 13-7-13 | 46:41 | 5 | 46 |
| 7. |  |  | VfB Lübeck | 33 | 10-15-8 | 38:34 | 4 | 45 |
| 8. | |  | Chemnitzer FC | 33 | 11-11-11 | 49:41 | 8 | 44 |
| 9. |  |  | SV Wilhelmshaven | 33 | 12-7-14 | 44:50 | -6 | 43 |
| 10. | |  | Hertha BSC Berlin II | 33 | 11-10-12 | 39:52 | -13 | 43 |
| 11. | |  | FC Oberneuland 1948 | 33 | 11-8-14 | 39:41 | -2 | 41 |
| 12. | |  | FC Hansa Rostock II | 33 | 11-7-15 | 50:58 | -8 | 40 |
| 13. | |  | Hamburger SV II | 33 | 10-9-14 | 42:42 | 0 | 39 |
| 14. | |  | VFC Plauen | 33 | 9-12-12 | 51:54 | -3 | 39 |
| 15. | |  | Türkiyemspor Berlin | 33 | 11-6-16 | 35:43 | -8 | 39 |
| 16. | |  | Altonaer FC 93 | 33 | 8-7-18 | 40:65 | -25 | 31 |
| 17. | |  | FC Sachsen Leipzig | 33 | 5-9-19 | 21:53 | -32 | 24 |
| 18. | |  | FC Energie Cottbus II | 33 | 5-8-20 | 23:51 | -28 | 23 |