| Fr., 02.10.2009 | FC Oberneuland 1948 - Chemnitzer FC | 0:3 |
| Fr., 02.10.2009 | Tennis Borussia Berlin - Hertha BSC Berlin II | 0:2 |
| Sa., 03.10.2009 | VfL Wolfsburg II - VfB Lübeck | 5:0 |
| Sa., 03.10.2009 | Türkiyemspor Berlin - Hannover 96 II | 2:1 |
| So., 04.10.2009 | VFC Plauen - SV Wilhelmshaven | 0:1 |
| So., 04.10.2009 | 1. FC Magdeburg - Goslarer SC 08 | 2:1 |
| So., 04.10.2009 | ZFC Meuselwitz - FC St. Pauli II | 0:0 |
| So., 04.10.2009 | FC Hansa Rostock II - SV Babelsberg 03 | 0:2 |
| Mo., 05.10.2009 | Hamburger SV II - Hallescher FC | 0:1 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | VfL Wolfsburg II | 10 | 6-3-1 | 22:4 | 18 | 21 |
| 2. | |  | SV Babelsberg 03 | 10 | 6-2-2 | 20:9 | 11 | 20 |
| 3. |  |  | Hallescher FC | 10 | 5-4-1 | 16:9 | 7 | 19 |
| 4. | |  | Chemnitzer FC | 10 | 5-3-2 | 19:11 | 8 | 18 |
| 5. |  |  | VfB Lübeck | 10 | 5-2-3 | 18:15 | 3 | 17 |
| 6. | |  | 1. FC Magdeburg | 10 | 4-4-2 | 21:14 | 7 | 16 |
| 7. | |  | Türkiyemspor Berlin | 10 | 5-1-4 | 15:11 | 4 | 16 |
| 8. | |  | Hertha BSC Berlin II | 10 | 5-1-4 | 14:13 | 1 | 16 |
| 9. | |  | Hannover 96 II | 10 | 4-2-4 | 15:11 | 4 | 14 |
| 10. | |  | Tennis Borussia Berlin | 10 | 4-2-4 | 9:11 | -2 | 14 |
| 11. | |  | FC St. Pauli II | 10 | 3-4-3 | 11:13 | -2 | 13 |
| 12. | |  | ZFC Meuselwitz | 10 | 2-5-3 | 9:10 | -1 | 11 |
| 13. | |  | SV Wilhelmshaven | 10 | 2-5-3 | 12:15 | -3 | 11 |
| 14. | |  | Hamburger SV II | 10 | 2-5-3 | 10:14 | -4 | 11 |
| 15. | |  | FC Oberneuland 1948 | 10 | 3-1-6 | 12:24 | -12 | 10 |
| 16. | |  | VFC Plauen | 10 | 2-3-5 | 9:16 | -7 | 9 |
| 17. | |  | FC Hansa Rostock II | 10 | 2-1-7 | 12:28 | -16 | 7 |
| 18. | |  | Goslarer SC 08 | 10 | 1-0-9 | 7:23 | -16 | 3 |