| Fr., 20.11.2009 | Tennis Borussia Berlin - ZFC Meuselwitz | 1:2 |
| Sa., 21.11.2009 | Hallescher FC - SV Babelsberg 03 | 0:1 |
| Sa., 21.11.2009 | VfB Lübeck - Hannover 96 II | 1:3 |
| Sa., 21.11.2009 | Goslarer SC 08 - SV Wilhelmshaven | 2:2 |
| So., 22.11.2009 | FC Oberneuland 1948 - 1. FC Magdeburg | 1:0 |
| So., 22.11.2009 | Chemnitzer FC - Türkiyemspor Berlin | 3:0 |
| So., 22.11.2009 | FC St. Pauli II - VFC Plauen | 3:2 |
| So., 22.11.2009 | VfL Wolfsburg II - Hamburger SV II | 0:0 |
| So., 22.11.2009 | Hertha BSC Berlin II - FC Hansa Rostock II | 2:3 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | SV Babelsberg 03 | 15 | 11-2-2 | 26:10 | 16 | 35 |
| 2. |  |  | VfL Wolfsburg II | 15 | 8-5-2 | 26:7 | 19 | 29 |
| 3. |  |  | Hallescher FC | 15 | 8-5-2 | 26:10 | 16 | 29 |
| 4. | |  | Chemnitzer FC | 15 | 8-4-3 | 29:16 | 13 | 28 |
| 5. | |  | 1. FC Magdeburg | 15 | 7-5-3 | 31:17 | 14 | 26 |
| 6. | |  | VfB Lübeck | 15 | 7-2-6 | 24:22 | 2 | 23 |
| 7. | |  | Türkiyemspor Berlin | 15 | 7-2-6 | 23:25 | -2 | 23 |
| 8. | |  | Hamburger SV II | 15 | 5-6-4 | 18:17 | 1 | 21 |
| 9. | |  | Tennis Borussia Berlin | 16 | 6-3-7 | 14:18 | -4 | 21 |
| 10. | |  | Hannover 96 II | 15 | 6-2-7 | 24:19 | 5 | 20 |
| 11. | |  | Hertha BSC Berlin II | 15 | 6-1-8 | 24:26 | -2 | 19 |
| 12. | |  | ZFC Meuselwitz | 15 | 4-6-5 | 18:21 | -3 | 18 |
| 13. | |  | FC St. Pauli II | 15 | 4-6-5 | 16:24 | -8 | 18 |
| 14. | |  | FC Oberneuland 1948 | 15 | 5-2-8 | 15:28 | -13 | 17 |
| 15. | |  | FC Hansa Rostock II | 16 | 4-3-9 | 18:34 | -16 | 15 |
| 16. | |  | VFC Plauen | 15 | 3-5-7 | 14:22 | -8 | 14 |
| 17. | |  | SV Wilhelmshaven | 15 | 2-6-7 | 17:29 | -12 | 12 |
| 18. | |  | Goslarer SC 08 | 15 | 1-3-11 | 14:32 | -18 | 6 |