| Fr., 27.11.2009 | FC Hansa Rostock II - Chemnitzer FC | 0:5 |
| Fr., 27.11.2009 | Hannover 96 II - Hallescher FC | 1:1 |
| Fr., 27.11.2009 | SV Wilhelmshaven - FC St. Pauli II | 2:1 |
| Sa., 28.11.2009 | SV Babelsberg 03 - Goslarer SC 08 | 1:1 |
| Sa., 28.11.2009 | Türkiyemspor Berlin - VfL Wolfsburg II | 0:3 |
| So., 29.11.2009 | VfB Lübeck - ZFC Meuselwitz | 0:1 |
| So., 29.11.2009 | VFC Plauen - Hertha BSC Berlin II | 2:1 |
| So., 29.11.2009 | 1. FC Magdeburg - Tennis Borussia Berlin | 7:0 |
| So., 29.11.2009 | Hamburger SV II - FC Oberneuland 1948 | 2:0 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | SV Babelsberg 03 | 16 | 11-3-2 | 27:11 | 16 | 36 |
| 2. | |  | VfL Wolfsburg II | 16 | 9-5-2 | 29:7 | 22 | 32 |
| 3. |  |  | Chemnitzer FC | 16 | 9-4-3 | 34:16 | 18 | 31 |
| 4. |  |  | Hallescher FC | 16 | 8-6-2 | 27:11 | 16 | 30 |
| 5. | |  | 1. FC Magdeburg | 16 | 8-5-3 | 38:17 | 21 | 29 |
| 6. | |  | Hamburger SV II | 16 | 6-6-4 | 20:17 | 3 | 24 |
| 7. | |  | VfB Lübeck | 16 | 7-2-7 | 24:23 | 1 | 23 |
| 8. | |  | Türkiyemspor Berlin | 16 | 7-2-7 | 23:28 | -5 | 23 |
| 9. | |  | Hannover 96 II | 16 | 6-3-7 | 25:20 | 5 | 21 |
| 10. | |  | ZFC Meuselwitz | 16 | 5-6-5 | 19:21 | -2 | 21 |
| 11. | |  | Tennis Borussia Berlin | 17 | 6-3-8 | 14:25 | -11 | 21 |
| 12. | |  | Hertha BSC Berlin II | 16 | 6-1-9 | 25:28 | -3 | 19 |
| 13. | |  | FC St. Pauli II | 16 | 4-6-6 | 17:26 | -9 | 18 |
| 14. | |  | VFC Plauen | 16 | 4-5-7 | 16:23 | -7 | 17 |
| 15. | |  | FC Oberneuland 1948 | 16 | 5-2-9 | 15:30 | -15 | 17 |
| 16. | |  | SV Wilhelmshaven | 16 | 3-6-7 | 19:30 | -11 | 15 |
| 17. | |  | FC Hansa Rostock II | 17 | 4-3-10 | 18:39 | -21 | 15 |
| 18. | |  | Goslarer SC 08 | 16 | 1-4-11 | 15:33 | -18 | 7 |