| Mi., 09.12.2009 | FC St. Pauli II - Hannover 96 II | 2:1 |
| Fr., 11.12.2009 | VfL Wolfsburg II - VFC Plauen | 1:0 |
| Sa., 12.12.2009 | Hertha BSC Berlin II - SV Babelsberg 03 | 1:4 |
| Sa., 12.12.2009 | Tennis Borussia Berlin - Türkiyemspor Berlin | 3:1 |
| So., 13.12.2009 | 1. FC Magdeburg - VfB Lübeck | 1:0 |
| So., 13.12.2009 | Chemnitzer FC - SV Wilhelmshaven | 5:1 |
| So., 13.12.2009 | FC Oberneuland 1948 - FC Hansa Rostock II | 2:2 |
| Mi., 17.03.2010 | ZFC Meuselwitz - Hamburger SV II | 2:1 |
| Mi., 21.04.2010 | Goslarer SC 08 - Hallescher FC | 0:0 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | SV Babelsberg 03 | 18 | 13-3-2 | 32:12 | 20 | 42 |
| 2. | |  | VfL Wolfsburg II | 18 | 11-5-2 | 31:7 | 24 | 38 |
| 3. |  |  | Chemnitzer FC | 18 | 10-4-4 | 40:20 | 20 | 34 |
| 4. |  |  | Hallescher FC | 17 | 9-6-2 | 31:11 | 20 | 33 |
| 5. | |  | 1. FC Magdeburg | 18 | 9-5-4 | 39:18 | 21 | 32 |
| 6. | |  | Hamburger SV II | 17 | 7-6-4 | 24:18 | 6 | 27 |
| 7. | |  | ZFC Meuselwitz | 17 | 6-6-5 | 20:21 | -1 | 24 |
| 8. | |  | Tennis Borussia Berlin | 19 | 7-3-9 | 18:30 | -12 | 24 |
| 9. | |  | VfB Lübeck | 18 | 7-2-9 | 24:28 | -4 | 23 |
| 10. | |  | Türkiyemspor Berlin | 18 | 7-2-9 | 25:34 | -9 | 23 |
| 11. | |  | Hannover 96 II | 18 | 6-3-9 | 27:24 | 3 | 21 |
| 12. | |  | FC St. Pauli II | 18 | 5-6-7 | 19:28 | -9 | 21 |
| 13. | |  | FC Oberneuland 1948 | 18 | 6-3-9 | 20:33 | -13 | 21 |
| 14. | |  | VFC Plauen | 18 | 5-5-8 | 19:25 | -6 | 20 |
| 15. | |  | Hertha BSC Berlin II | 18 | 6-1-11 | 27:35 | -8 | 19 |
| 16. | |  | SV Wilhelmshaven | 18 | 4-6-8 | 23:36 | -13 | 18 |
| 17. | |  | FC Hansa Rostock II | 19 | 4-4-11 | 20:42 | -22 | 16 |
| 18. | |  | Goslarer SC 08 | 17 | 2-4-11 | 17:34 | -17 | 10 |