| Sa., 27.03.2010 | VFC Plauen - VfB Lübeck | 0:1 |
| Sa., 27.03.2010 | Hamburger SV II - Hannover 96 II | 1:1 |
| Sa., 27.03.2010 | Türkiyemspor Berlin - SV Babelsberg 03 | 0:1 |
| Sa., 27.03.2010 | FC Hansa Rostock II - SV Wilhelmshaven | 2:1 |
| Sa., 27.03.2010 | Tennis Borussia Berlin - FC St. Pauli II | 2:1 |
| So., 28.03.2010 | VfL Wolfsburg II - Chemnitzer FC | 0:1 |
| So., 28.03.2010 | ZFC Meuselwitz - Goslarer SC 08 | 1:1 |
| So., 28.03.2010 | FC Oberneuland 1948 - Hertha BSC Berlin II | 1:1 |
| So., 28.03.2010 | 1. FC Magdeburg - Hallescher FC | 0:1 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | SV Babelsberg 03 | 25 | 16-7-2 | 38:13 | 25 | 55 |
| 2. | |  | Hallescher FC | 24 | 13-9-2 | 39:14 | 25 | 48 |
| 3. | |  | VfL Wolfsburg II | 23 | 13-6-4 | 35:12 | 23 | 45 |
| 4. | |  | Chemnitzer FC | 24 | 13-6-5 | 46:24 | 22 | 45 |
| 5. | |  | 1. FC Magdeburg | 25 | 9-7-9 | 42:28 | 14 | 34 |
| 6. |  |  | VfB Lübeck | 24 | 10-4-10 | 32:32 | 0 | 34 |
| 7. |  |  | Hannover 96 II | 24 | 9-5-10 | 40:31 | 9 | 32 |
| 8. | |  | FC Hansa Rostock II | 25 | 9-5-11 | 31:46 | -15 | 32 |
| 9. | |  | Tennis Borussia Berlin | 25 | 8-7-10 | 24:37 | -13 | 31 |
| 10. | |  | Hamburger SV II | 21 | 7-9-5 | 27:22 | 5 | 30 |
| 11. | |  | VFC Plauen | 24 | 8-6-10 | 27:32 | -5 | 30 |
| 12. | |  | ZFC Meuselwitz | 23 | 7-9-7 | 26:31 | -5 | 30 |
| 13. | |  | FC Oberneuland 1948 | 24 | 7-6-11 | 28:42 | -14 | 27 |
| 14. | |  | FC St. Pauli II | 23 | 6-8-9 | 26:36 | -10 | 26 |
| 15. | |  | Türkiyemspor Berlin | 24 | 7-5-12 | 32:46 | -14 | 26 |
| 16. | |  | Hertha BSC Berlin II | 24 | 6-6-12 | 36:46 | -10 | 24 |
| 17. | |  | SV Wilhelmshaven | 21 | 4-7-10 | 25:40 | -15 | 19 |
| 18. | |  | Goslarer SC 08 | 23 | 2-6-15 | 21:43 | -22 | 12 |