| Fr., 30.04.2010 | Chemnitzer FC - Hamburger SV II | 1:0 |
| Fr., 30.04.2010 | VfB Lübeck - Tennis Borussia Berlin | 3:0 |
| Sa., 01.05.2010 | Goslarer SC 08 - VFC Plauen | 1:2 |
| Sa., 01.05.2010 | FC Oberneuland 1948 - ZFC Meuselwitz | 2:0 |
| Sa., 01.05.2010 | Hertha BSC Berlin II - Türkiyemspor Berlin | 5:1 |
| So., 02.05.2010 | Hannover 96 II - SV Babelsberg 03 | 1:2 |
| So., 02.05.2010 | Hallescher FC - SV Wilhelmshaven | 1:2 |
| So., 02.05.2010 | FC St. Pauli II - FC Hansa Rostock II | 1:2 |
| So., 02.05.2010 | VfL Wolfsburg II - 1. FC Magdeburg | 1:0 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | SV Babelsberg 03 | 31 | 21-8-2 | 49:15 | 34 | 71 |
| 2. | |  | VfL Wolfsburg II | 31 | 20-7-4 | 58:15 | 43 | 67 |
| 3. |  |  | Chemnitzer FC | 31 | 16-7-8 | 55:32 | 23 | 55 |
| 4. |  |  | Hallescher FC | 31 | 14-12-5 | 45:22 | 23 | 54 |
| 5. | |  | VfB Lübeck | 31 | 14-5-12 | 43:41 | 2 | 47 |
| 6. | |  | Hamburger SV II | 31 | 12-10-9 | 39:32 | 7 | 46 |
| 7. | |  | 1. FC Magdeburg | 31 | 12-8-11 | 47:34 | 13 | 44 |
| 8. | |  | VFC Plauen | 31 | 12-8-11 | 40:37 | 3 | 44 |
| 9. | |  | Hannover 96 II | 31 | 11-9-11 | 54:39 | 15 | 42 |
| 10. | |  | Hertha BSC Berlin II | 31 | 11-7-13 | 55:51 | 4 | 40 |
| 11. | |  | ZFC Meuselwitz | 31 | 10-10-11 | 34:44 | -10 | 40 |
| 12. | |  | FC Hansa Rostock II | 31 | 11-6-14 | 36:58 | -22 | 39 |
| 13. | |  | Tennis Borussia Berlin | 31 | 8-9-14 | 27:47 | -20 | 33 |
| 14. | |  | FC Oberneuland 1948 | 31 | 9-6-16 | 36:59 | -23 | 33 |
| 15. | |  | SV Wilhelmshaven | 31 | 8-8-15 | 38:57 | -19 | 32 |
| 16. | |  | Türkiyemspor Berlin | 31 | 7-9-15 | 41:62 | -21 | 30 |
| 17. | |  | FC St. Pauli II | 31 | 7-9-15 | 33:54 | -21 | 30 |
| 18. | |  | Goslarer SC 08 | 31 | 3-8-20 | 29:60 | -31 | 17 |