| Fr., 07.05.2010 | Türkiyemspor Berlin - Chemnitzer FC | 2:1 |
| Sa., 08.05.2010 | ZFC Meuselwitz - Tennis Borussia Berlin | 2:2 |
| Sa., 08.05.2010 | Hannover 96 II - VfB Lübeck | 3:0 |
| Sa., 08.05.2010 | Hamburger SV II - VfL Wolfsburg II | 2:0 |
| Sa., 08.05.2010 | FC Hansa Rostock II - Hertha BSC Berlin II | 0:0 |
| Sa., 08.05.2010 | SV Wilhelmshaven - Goslarer SC 08 | 3:1 |
| So., 09.05.2010 | 1. FC Magdeburg - FC Oberneuland 1948 | 5:0 |
| So., 09.05.2010 | VFC Plauen - FC St. Pauli II | 6:0 |
| So., 09.05.2010 | SV Babelsberg 03 - Hallescher FC | 1:0 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | SV Babelsberg 03 | 32 | 22-8-2 | 50:15 | 35 | 74 |
| 2. | |  | VfL Wolfsburg II | 32 | 20-7-5 | 58:17 | 41 | 67 |
| 3. | |  | Chemnitzer FC | 32 | 16-7-9 | 56:34 | 22 | 55 |
| 4. | |  | Hallescher FC | 32 | 14-12-6 | 45:23 | 22 | 54 |
| 5. |  |  | Hamburger SV II | 32 | 13-10-9 | 41:32 | 9 | 49 |
| 6. | |  | 1. FC Magdeburg | 32 | 13-8-11 | 52:34 | 18 | 47 |
| 7. | |  | VFC Plauen | 32 | 13-8-11 | 46:37 | 9 | 47 |
| 8. |  |  | VfB Lübeck | 32 | 14-5-13 | 43:44 | -1 | 47 |
| 9. | |  | Hannover 96 II | 32 | 12-9-11 | 57:39 | 18 | 45 |
| 10. | |  | Hertha BSC Berlin II | 32 | 11-8-13 | 55:51 | 4 | 41 |
| 11. | |  | ZFC Meuselwitz | 32 | 10-11-11 | 36:46 | -10 | 41 |
| 12. | |  | FC Hansa Rostock II | 32 | 11-7-14 | 36:58 | -22 | 40 |
| 13. | |  | SV Wilhelmshaven | 32 | 9-8-15 | 41:58 | -17 | 35 |
| 14. | |  | Tennis Borussia Berlin | 32 | 8-10-14 | 29:49 | -20 | 34 |
| 15. | |  | Türkiyemspor Berlin | 32 | 8-9-15 | 43:63 | -20 | 33 |
| 16. | |  | FC Oberneuland 1948 | 32 | 9-6-17 | 36:64 | -28 | 33 |
| 17. | |  | FC St. Pauli II | 32 | 7-9-16 | 33:60 | -27 | 30 |
| 18. | |  | Goslarer SC 08 | 32 | 3-8-21 | 30:63 | -33 | 17 |