| Fr., 28.08.2009 | Tennis Borussia Berlin - SV Wilhelmshaven | 1:1 |
| Fr., 28.08.2009 | Chemnitzer FC - Hallescher FC | 1:1 |
| Fr., 28.08.2009 | FC St. Pauli II - VfB Lübeck | 1:3 |
| Sa., 29.08.2009 | 1. FC Magdeburg - FC Hansa Rostock II | 2:2 |
| Sa., 29.08.2009 | FC Oberneuland 1948 - SV Babelsberg 03 | 1:2 |
| Sa., 29.08.2009 | VfL Wolfsburg II - Hannover 96 II | 2:0 |
| Sa., 29.08.2009 | Hertha BSC Berlin II - Goslarer SC 08 | 2:1 |
| Sa., 29.08.2009 | Hamburger SV II - Türkiyemspor Berlin | 1:2 |
| So., 30.08.2009 | ZFC Meuselwitz - VFC Plauen | 0:0 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | VfL Wolfsburg II | 4 | 3-1-0 | 8:0 | 8 | 10 |
| 2. |  |  | Türkiyemspor Berlin | 4 | 3-0-1 | 6:4 | 2 | 9 |
| 3. |  |  | Hallescher FC | 4 | 2-2-0 | 8:4 | 4 | 8 |
| 4. | |  | VfB Lübeck | 4 | 2-2-0 | 7:3 | 4 | 8 |
| 5. | |  | Tennis Borussia Berlin | 4 | 2-1-1 | 6:4 | 2 | 7 |
| 6. | |  | FC Hansa Rostock II | 4 | 2-1-1 | 8:8 | 0 | 7 |
| 7. | |  | Hannover 96 II | 3 | 2-0-1 | 4:2 | 2 | 6 |
| 8. | |  | ZFC Meuselwitz | 4 | 1-2-1 | 5:4 | 1 | 5 |
| 9. | |  | SV Babelsberg 03 | 4 | 1-2-1 | 3:3 | 0 | 5 |
| 10. | |  | Chemnitzer FC | 4 | 1-2-1 | 5:6 | -1 | 5 |
| 11. | |  | FC Oberneuland 1948 | 4 | 1-1-2 | 8:9 | -1 | 4 |
| 12. | |  | Hertha BSC Berlin II | 4 | 1-1-2 | 4:6 | -2 | 4 |
| 13. | |  | 1. FC Magdeburg | 4 | 0-3-1 | 3:4 | -1 | 3 |
| 14. | |  | Hamburger SV II | 4 | 0-3-1 | 2:3 | -1 | 3 |
| 15. | |  | Goslarer SC 08 | 4 | 1-0-3 | 5:10 | -5 | 3 |
| 16. | |  | SV Wilhelmshaven | 4 | 0-2-2 | 5:8 | -3 | 2 |
| 17. | |  | VFC Plauen | 4 | 0-2-2 | 2:8 | -6 | 2 |
| 18. | |  | FC St. Pauli II | 3 | 0-1-2 | 4:7 | -3 | 1 |