| Fr., 04.09.2009 | Türkiyemspor Berlin - FC Hansa Rostock II | 5:1 |
| Sa., 05.09.2009 | FC Oberneuland 1948 - Hallescher FC | 1:0 |
| Sa., 05.09.2009 | Tennis Borussia Berlin - Hannover 96 II | 0:2 |
| So., 06.09.2009 | ZFC Meuselwitz - SV Babelsberg 03 | 0:3 |
| So., 06.09.2009 | Hamburger SV II - VFC Plauen | 1:1 |
| So., 06.09.2009 | 1. FC Magdeburg - SV Wilhelmshaven | 5:2 |
| So., 06.09.2009 | Hertha BSC Berlin II - VfB Lübeck | 1:0 |
| Mo., 07.09.2009 | Chemnitzer FC - FC St. Pauli II | 4:0 |
| Di., 08.09.2009 | VfL Wolfsburg II - Goslarer SC 08 | 4:0 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | VfL Wolfsburg II | 6 | 4-2-0 | 14:2 | 12 | 14 |
| 2. |  |  | Hannover 96 II | 5 | 4-0-1 | 11:2 | 9 | 12 |
| 3. | |  | Türkiyemspor Berlin | 6 | 4-0-2 | 11:6 | 5 | 12 |
| 4. | |  | Chemnitzer FC | 6 | 3-2-1 | 11:6 | 5 | 11 |
| 5. |  |  | VfB Lübeck | 6 | 3-2-1 | 8:4 | 4 | 11 |
| 6. | |  | Tennis Borussia Berlin | 6 | 3-1-2 | 7:6 | 1 | 10 |
| 7. | |  | 1. FC Magdeburg | 6 | 2-3-1 | 12:7 | 5 | 9 |
| 8. | |  | Hallescher FC | 6 | 2-3-1 | 10:7 | 3 | 9 |
| 9. | |  | SV Babelsberg 03 | 6 | 2-2-2 | 6:4 | 2 | 8 |
| 10. | |  | Hamburger SV II | 6 | 1-4-1 | 5:5 | 0 | 7 |
| 11. | |  | Hertha BSC Berlin II | 6 | 2-1-3 | 6:9 | -3 | 7 |
| 12. | |  | FC Hansa Rostock II | 6 | 2-1-3 | 10:15 | -5 | 7 |
| 13. | |  | FC Oberneuland 1948 | 6 | 2-1-3 | 9:14 | -5 | 7 |
| 14. | |  | ZFC Meuselwitz | 6 | 1-3-2 | 5:7 | -2 | 6 |
| 15. | |  | FC St. Pauli II | 5 | 1-1-3 | 7:12 | -5 | 4 |
| 16. | |  | SV Wilhelmshaven | 6 | 0-3-3 | 7:13 | -6 | 3 |
| 17. | |  | VFC Plauen | 6 | 0-3-3 | 4:13 | -9 | 3 |
| 18. | |  | Goslarer SC 08 | 6 | 1-0-5 | 5:16 | -11 | 3 |