| Fr., 18.09.2009 | Tennis Borussia Berlin - Goslarer SC 08 | 1:0 |
| Sa., 19.09.2009 | Chemnitzer FC - VfB Lübeck | 3:2 |
| Sa., 19.09.2009 | 1. FC Magdeburg - Hannover 96 II | 4:2 |
| Sa., 19.09.2009 | FC Oberneuland 1948 - FC St. Pauli II | 0:2 |
| Sa., 19.09.2009 | VfL Wolfsburg II - Hertha BSC Berlin II | 2:0 |
| Sa., 19.09.2009 | Hamburger SV II - SV Babelsberg 03 | 1:6 |
| So., 20.09.2009 | ZFC Meuselwitz - Hallescher FC | 1:2 |
| So., 20.09.2009 | FC Hansa Rostock II - VFC Plauen | 0:3 |
| So., 20.09.2009 | Türkiyemspor Berlin - SV Wilhelmshaven | 0:0 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | VfL Wolfsburg II | 8 | 5-2-1 | 16:3 | 13 | 17 |
| 2. |  |  | Hallescher FC | 8 | 4-3-1 | 14:8 | 6 | 15 |
| 3. | |  | SV Babelsberg 03 | 8 | 4-2-2 | 15:7 | 8 | 14 |
| 4. |  |  | VfB Lübeck | 8 | 4-2-2 | 16:9 | 7 | 14 |
| 5. | |  | Hannover 96 II | 8 | 4-2-2 | 14:7 | 7 | 14 |
| 6. | |  | Chemnitzer FC | 8 | 4-2-2 | 15:10 | 5 | 14 |
| 7. | |  | Türkiyemspor Berlin | 8 | 4-1-3 | 11:7 | 4 | 13 |
| 8. | |  | Tennis Borussia Berlin | 8 | 4-1-3 | 8:8 | 0 | 13 |
| 9. | |  | 1. FC Magdeburg | 8 | 3-3-2 | 18:12 | 6 | 12 |
| 10. | |  | FC St. Pauli II | 8 | 3-2-3 | 10:12 | -2 | 11 |
| 11. | |  | Hertha BSC Berlin II | 8 | 3-1-4 | 8:12 | -4 | 10 |
| 12. | |  | FC Oberneuland 1948 | 8 | 3-1-4 | 11:17 | -6 | 10 |
| 13. | |  | VFC Plauen | 8 | 2-3-3 | 8:13 | -5 | 9 |
| 14. | |  | Hamburger SV II | 8 | 1-5-2 | 8:13 | -5 | 8 |
| 15. | |  | ZFC Meuselwitz | 8 | 1-4-3 | 7:10 | -3 | 7 |
| 16. | |  | FC Hansa Rostock II | 8 | 2-1-5 | 12:24 | -12 | 7 |
| 17. | |  | SV Wilhelmshaven | 8 | 0-5-3 | 9:15 | -6 | 5 |
| 18. | |  | Goslarer SC 08 | 8 | 1-0-7 | 6:19 | -13 | 3 |