| Fr., 06.11.2009 | FC Hansa Rostock II - FC St. Pauli II | 0:0 |
| Fr., 06.11.2009 | Türkiyemspor Berlin - Hertha BSC Berlin II | 3:1 |
| Fr., 06.11.2009 | SV Wilhelmshaven - Hallescher FC | 0:2 |
| Sa., 07.11.2009 | Tennis Borussia Berlin - VfB Lübeck | 3:0 |
| Sa., 07.11.2009 | SV Babelsberg 03 - Hannover 96 II | 1:0 |
| So., 08.11.2009 | VFC Plauen - Goslarer SC 08 | 1:1 |
| So., 08.11.2009 | Hamburger SV II - Chemnitzer FC | 1:2 |
| So., 08.11.2009 | 1. FC Magdeburg - VfL Wolfsburg II | 3:1 |
| So., 08.11.2009 | ZFC Meuselwitz - FC Oberneuland 1948 | 1:2 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | SV Babelsberg 03 | 14 | 10-2-2 | 25:10 | 15 | 32 |
| 2. |  |  | Hallescher FC | 14 | 8-5-1 | 26:9 | 17 | 29 |
| 3. |  |  | VfL Wolfsburg II | 14 | 8-4-2 | 26:7 | 19 | 28 |
| 4. | |  | 1. FC Magdeburg | 14 | 7-5-2 | 31:16 | 15 | 26 |
| 5. | |  | Chemnitzer FC | 14 | 7-4-3 | 26:16 | 10 | 25 |
| 6. | |  | VfB Lübeck | 14 | 7-2-5 | 23:19 | 4 | 23 |
| 7. | |  | Türkiyemspor Berlin | 14 | 7-2-5 | 23:22 | 1 | 23 |
| 8. | |  | Tennis Borussia Berlin | 15 | 6-3-6 | 13:16 | -3 | 21 |
| 9. | |  | Hamburger SV II | 14 | 5-5-4 | 18:17 | 1 | 20 |
| 10. | |  | Hertha BSC Berlin II | 14 | 6-1-7 | 22:23 | -1 | 19 |
| 11. | |  | Hannover 96 II | 14 | 5-2-7 | 21:18 | 3 | 17 |
| 12. | |  | ZFC Meuselwitz | 14 | 3-6-5 | 16:20 | -4 | 15 |
| 13. | |  | FC St. Pauli II | 14 | 3-6-5 | 13:22 | -9 | 15 |
| 14. | |  | VFC Plauen | 14 | 3-5-6 | 12:19 | -7 | 14 |
| 15. | |  | FC Oberneuland 1948 | 14 | 4-2-8 | 14:28 | -14 | 14 |
| 16. | |  | FC Hansa Rostock II | 15 | 3-3-9 | 15:32 | -17 | 12 |
| 17. | |  | SV Wilhelmshaven | 14 | 2-5-7 | 15:27 | -12 | 11 |
| 18. | |  | Goslarer SC 08 | 14 | 1-2-11 | 12:30 | -18 | 5 |