| Sa., 28.02.2009 | FC Sachsen Leipzig - Hertha BSC Berlin II | 1:1 |
| Sa., 28.02.2009 | VfB Lübeck - Hamburger SV II | 1:0 |
| Sa., 28.02.2009 | 1. FC Magdeburg - SV Babelsberg 03 | 1:1 |
| Sa., 28.02.2009 | FC Energie Cottbus II - Holstein Kiel | 1:2 |
| Sa., 28.02.2009 | Altonaer FC 93 - Hannover 96 II | 0:3 |
| So., 01.03.2009 | SV Wilhelmshaven - Hallescher FC | 0:1 |
| Mi., 01.04.2009 | FC Oberneuland 1948 - FC Hansa Rostock II | 3:0 |
| Mi., 01.04.2009 | VFC Plauen - VfL Wolfsburg II | 1:5 |
| Mi., 22.04.2009 | Türkiyemspor Berlin - Chemnitzer FC | 0:3 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | Holstein Kiel | 20 | 13-5-2 | 33:16 | 17 | 44 |
| 2. |  |  | Hallescher FC | 20 | 11-9-0 | 23:8 | 15 | 42 |
| 3. |  |  | 1. FC Magdeburg | 20 | 12-5-3 | 31:15 | 16 | 41 |
| 4. | |  | SV Babelsberg 03 | 19 | 9-9-1 | 21:9 | 12 | 36 |
| 5. | |  | SV Wilhelmshaven | 19 | 8-6-5 | 27:21 | 6 | 30 |
| 6. | |  | Hannover 96 II | 20 | 9-3-8 | 29:24 | 5 | 30 |
| 7. | |  | FC Hansa Rostock II | 18 | 8-2-8 | 31:28 | 3 | 26 |
| 8. | |  | VfL Wolfsburg II | 18 | 7-4-7 | 23:17 | 6 | 25 |
| 9. | |  | VFC Plauen | 18 | 6-6-6 | 25:25 | 0 | 24 |
| 10. | |  | VfB Lübeck | 20 | 5-9-6 | 20:20 | 0 | 24 |
| 11. | |  | Chemnitzer FC | 18 | 6-5-7 | 27:23 | 4 | 23 |
| 12. | |  | FC Oberneuland 1948 | 18 | 6-4-8 | 17:19 | -2 | 22 |
| 13. | |  | Altonaer FC 93 | 19 | 6-4-9 | 24:33 | -9 | 22 |
| 14. | |  | Türkiyemspor Berlin | 18 | 6-2-10 | 17:25 | -8 | 20 |
| 15. | |  | Hertha BSC Berlin II | 19 | 5-5-9 | 19:33 | -14 | 20 |
| 16. | |  | Hamburger SV II | 19 | 4-5-10 | 19:26 | -7 | 17 |
| 17. | |  | FC Sachsen Leipzig | 19 | 2-7-10 | 9:27 | -18 | 13 |
| 18. | |  | FC Energie Cottbus II | 20 | 1-4-15 | 12:38 | -26 | 7 |