| Sa., 29.05.2010 | 1. FC Magdeburg - ZFC Meuselwitz | 1:1 |
| Sa., 29.05.2010 | Türkiyemspor Berlin - FC Oberneuland 1948 | 5:0 |
| Sa., 29.05.2010 | Hamburger SV II - Tennis Borussia Berlin | 2:1 |
| Sa., 29.05.2010 | FC Hansa Rostock II - VfL Wolfsburg II | 0:1 |
| Sa., 29.05.2010 | VFC Plauen - Chemnitzer FC | 0:1 |
| Sa., 29.05.2010 | SV Wilhelmshaven - Hertha BSC Berlin II | 1:1 |
| Sa., 29.05.2010 | SV Babelsberg 03 - FC St. Pauli II | 4:1 |
| Sa., 29.05.2010 | Hannover 96 II - Goslarer SC 08 | 4:1 |
| Sa., 29.05.2010 | VfB Lübeck - Hallescher FC | 1:1 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | SV Babelsberg 03 | 34 | 23-8-3 | 54:18 | 36 | 77 |
| 2. | |  | VfL Wolfsburg II | 34 | 21-7-6 | 60:20 | 40 | 70 |
| 3. | |  | Chemnitzer FC | 34 | 18-7-9 | 58:34 | 24 | 61 |
| 4. | |  | Hallescher FC | 34 | 14-14-6 | 47:25 | 22 | 56 |
| 5. | |  | Hamburger SV II | 34 | 15-10-9 | 45:34 | 11 | 55 |
| 6. | |  | 1. FC Magdeburg | 34 | 14-9-11 | 57:38 | 19 | 51 |
| 7. | |  | VFC Plauen | 34 | 14-8-12 | 49:39 | 10 | 50 |
| 8. |  |  | Hannover 96 II | 34 | 13-10-11 | 62:41 | 21 | 49 |
| 9. |  |  | VfB Lübeck | 34 | 14-7-13 | 47:48 | -1 | 49 |
| 10. | |  | ZFC Meuselwitz | 34 | 10-13-11 | 40:50 | -10 | 43 |
| 11. | |  | Hertha BSC Berlin II | 34 | 11-9-14 | 57:55 | 2 | 42 |
| 12. | |  | FC Hansa Rostock II | 34 | 11-7-16 | 36:60 | -24 | 40 |
| 13. | |  | Türkiyemspor Berlin | 34 | 10-9-15 | 51:64 | -13 | 39 |
| 14. | |  | SV Wilhelmshaven | 34 | 10-9-15 | 45:59 | -14 | 39 |
| 15. | |  | Tennis Borussia Berlin | 34 | 8-10-16 | 33:55 | -22 | 34 |
| 16. | |  | FC Oberneuland 1948 | 34 | 9-6-19 | 37:71 | -34 | 33 |
| 17. | |  | FC St. Pauli II | 34 | 7-9-18 | 34:67 | -33 | 30 |
| 18. | |  | Goslarer SC 08 | 34 | 4-8-22 | 33:67 | -34 | 20 |