| Di., 23.02.2010 | 1. FC Magdeburg - VFC Plauen | 0:1 |
| So., 28.02.2010 | Chemnitzer FC - Goslarer SC 08 | 1:0 |
| Di., 09.03.2010 | Tennis Borussia Berlin - SV Babelsberg 03 | 1:1 |
| Mi., 07.04.2010 | FC Oberneuland 1948 - Hannover 96 II | 1:3 |
| Mi., 07.04.2010 | Hamburger SV II - FC Hansa Rostock II | 3:0 |
| Mi., 07.04.2010 | Türkiyemspor Berlin - VfB Lübeck | 1:1 |
| Mi., 14.04.2010 | VfL Wolfsburg II - Hallescher FC | 1:1 |
| Mi., 21.04.2010 | ZFC Meuselwitz - SV Wilhelmshaven | 0:4 |
| Mi., 28.04.2010 | Hertha BSC Berlin II - FC St. Pauli II | 3:0 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | SV Babelsberg 03 | 18 | 13-3-2 | 32:12 | 20 | 42 |
| 2. |  |  | Chemnitzer FC | 20 | 12-4-4 | 43:20 | 23 | 40 |
| 3. |  |  | VfL Wolfsburg II | 19 | 11-5-3 | 31:10 | 21 | 38 |
| 4. | |  | Hallescher FC | 18 | 10-6-2 | 32:11 | 21 | 36 |
| 5. | |  | 1. FC Magdeburg | 21 | 9-6-6 | 40:21 | 19 | 33 |
| 6. | |  | Hamburger SV II | 17 | 7-6-4 | 24:18 | 6 | 27 |
| 7. | |  | VFC Plauen | 20 | 7-5-8 | 21:25 | -4 | 26 |
| 8. | |  | Hannover 96 II | 21 | 7-4-10 | 33:29 | 4 | 25 |
| 9. | |  | ZFC Meuselwitz | 17 | 6-6-5 | 20:21 | -1 | 24 |
| 10. | |  | Türkiyemspor Berlin | 19 | 7-3-9 | 26:35 | -9 | 24 |
| 11. | |  | FC St. Pauli II | 20 | 6-6-8 | 21:30 | -9 | 24 |
| 12. | |  | Tennis Borussia Berlin | 19 | 7-3-9 | 18:30 | -12 | 24 |
| 13. | |  | VfB Lübeck | 18 | 7-2-9 | 24:28 | -4 | 23 |
| 14. | |  | FC Oberneuland 1948 | 19 | 6-3-10 | 20:34 | -14 | 21 |
| 15. | |  | Hertha BSC Berlin II | 19 | 6-2-11 | 30:38 | -8 | 20 |
| 16. | |  | FC Hansa Rostock II | 20 | 5-4-11 | 21:42 | -21 | 19 |
| 17. | |  | SV Wilhelmshaven | 18 | 4-6-8 | 23:36 | -13 | 18 |
| 18. | |  | Goslarer SC 08 | 19 | 2-4-13 | 18:37 | -19 | 10 |