| Fr., 11.09.2009 | Hannover 96 II - ZFC Meuselwitz | 1:1 |
| Fr., 11.09.2009 | Hertha BSC Berlin II - Chemnitzer FC | 2:1 |
| Fr., 11.09.2009 | Goslarer SC 08 - FC Oberneuland 1948 | 1:2 |
| Sa., 12.09.2009 | SV Babelsberg 03 - 1. FC Magdeburg | 3:2 |
| Sa., 12.09.2009 | VfB Lübeck - FC Hansa Rostock II | 6:2 |
| Sa., 12.09.2009 | SV Wilhelmshaven - Hamburger SV II | 2:2 |
| So., 13.09.2009 | VFC Plauen - Türkiyemspor Berlin | 1:0 |
| So., 13.09.2009 | Hallescher FC - Tennis Borussia Berlin | 2:0 |
| So., 13.09.2009 | FC St. Pauli II - VfL Wolfsburg II | 1:0 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | VfL Wolfsburg II | 7 | 4-2-1 | 14:3 | 11 | 14 |
| 2. | |  | Hannover 96 II | 7 | 4-2-1 | 12:3 | 9 | 14 |
| 3. |  |  | VfB Lübeck | 7 | 4-2-1 | 14:6 | 8 | 14 |
| 4. | |  | Hallescher FC | 7 | 3-3-1 | 12:7 | 5 | 12 |
| 5. |  |  | Türkiyemspor Berlin | 7 | 4-0-3 | 11:7 | 4 | 12 |
| 6. | |  | Chemnitzer FC | 7 | 3-2-2 | 12:8 | 4 | 11 |
| 7. | |  | SV Babelsberg 03 | 7 | 3-2-2 | 9:6 | 3 | 11 |
| 8. | |  | Tennis Borussia Berlin | 7 | 3-1-3 | 7:8 | -1 | 10 |
| 9. | |  | Hertha BSC Berlin II | 7 | 3-1-3 | 8:10 | -2 | 10 |
| 10. | |  | FC Oberneuland 1948 | 7 | 3-1-3 | 11:15 | -4 | 10 |
| 11. | |  | 1. FC Magdeburg | 7 | 2-3-2 | 14:10 | 4 | 9 |
| 12. | |  | Hamburger SV II | 7 | 1-5-1 | 7:7 | 0 | 8 |
| 13. | |  | FC St. Pauli II | 7 | 2-2-3 | 8:12 | -4 | 8 |
| 14. | |  | ZFC Meuselwitz | 7 | 1-4-2 | 6:8 | -2 | 7 |
| 15. | |  | FC Hansa Rostock II | 7 | 2-1-4 | 12:21 | -9 | 7 |
| 16. | |  | VFC Plauen | 7 | 1-3-3 | 5:13 | -8 | 6 |
| 17. | |  | SV Wilhelmshaven | 7 | 0-4-3 | 9:15 | -6 | 4 |
| 18. | |  | Goslarer SC 08 | 7 | 1-0-6 | 6:18 | -12 | 3 |