| Fr., 28.04.2000 | FC St. Pauli - Hannover 96 | 0:2 |
| Fr., 28.04.2000 | Rot-Weiß Oberhausen - 1. FC Nürnberg | 1:0 |
| Fr., 28.04.2000 | VfL Bochum - Kickers Offenbach | 6:1 |
| Sa., 29.04.2000 | Tennis Borussia Berlin - SV Waldhof Mannheim | 3:1 |
| So., 30.04.2000 | 1. FC Köln - Chemnitzer FC | 2:1 |
| So., 30.04.2000 | Stuttgarter Kickers - SC Fortuna Köln | 2:1 |
| So., 30.04.2000 | Alemannia Aachen - Karlsruher SC | 4:1 |
| So., 30.04.2000 | SpVgg Greuther Fürth - FSV Mainz 05 | 2:0 |
| Mo., 01.05.2000 | FC Energie Cottbus - Borussia Mönchengladbach | 2:2 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | 1. FC Köln | 29 | 16-8-5 | 54:28 | 26 | 56 |
| 2. | |  | VfL Bochum | 29 | 15-6-8 | 55:41 | 14 | 51 |
| 3. | |  | FC Energie Cottbus | 29 | 14-4-11 | 52:36 | 16 | 46 |
| 4. |  |  | Borussia Mönchengladbach | 29 | 11-12-6 | 49:37 | 12 | 45 |
| 5. |  |  | 1. FC Nürnberg | 29 | 12-9-8 | 44:41 | 3 | 45 |
| 6. | |  | Hannover 96 | 29 | 12-7-10 | 47:42 | 5 | 43 |
| 7. | |  | Alemannia Aachen | 29 | 11-9-9 | 41:45 | -4 | 42 |
| 8. | |  | Tennis Borussia Berlin | 29 | 10-10-9 | 38:40 | -2 | 40 |
| 9. | |  | SV Waldhof Mannheim | 29 | 9-12-8 | 43:44 | -1 | 39 |
| 10. | |  | Rot-Weiß Oberhausen | 29 | 9-11-9 | 33:31 | 2 | 38 |
| 11. | |  | SpVgg Greuther Fürth | 29 | 8-14-7 | 32:33 | -1 | 38 |
| 12. | |  | Chemnitzer FC | 29 | 9-8-12 | 37:42 | -5 | 35 |
| 13. | |  | Stuttgarter Kickers | 29 | 9-7-13 | 45:50 | -5 | 34 |
| 14. | |  | FC St. Pauli | 29 | 7-13-9 | 29:34 | -5 | 34 |
| 15. | |  | FSV Mainz 05 | 29 | 7-12-10 | 33:35 | -2 | 33 |
| 16. | |  | Kickers Offenbach | 29 | 6-11-12 | 28:48 | -20 | 29 |
| 17. | |  | SC Fortuna Köln | 29 | 5-11-13 | 26:42 | -16 | 26 |
| 18. | |  | Karlsruher SC | 29 | 4-10-15 | 32:49 | -17 | 22 |