| Sa., 06.06.2009 | SV Babelsberg 03 - FC Sachsen Leipzig | 1:0 |
| So., 07.06.2009 | Chemnitzer FC - 1. FC Magdeburg | 1:1 |
| So., 07.06.2009 | Türkiyemspor Berlin - Altonaer FC 93 | 1:0 |
| So., 07.06.2009 | FC Oberneuland 1948 - FC Energie Cottbus II | 2:0 |
| So., 07.06.2009 | Hannover 96 II - SV Wilhelmshaven | 2:1 |
| So., 07.06.2009 | Holstein Kiel - VfB Lübeck | 1:0 |
| So., 07.06.2009 | Hallescher FC - VFC Plauen | 0:1 |
| So., 07.06.2009 | Hamburger SV II - VfL Wolfsburg II | 2:1 |
| So., 07.06.2009 | FC Hansa Rostock II - Hertha BSC Berlin II | 3:1 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | Holstein Kiel | 34 | 21-10-3 | 54:22 | 32 | 73 |
| 2. | |  | Hallescher FC | 34 | 19-13-2 | 43:20 | 23 | 70 |
| 3. | |  | SV Babelsberg 03 | 34 | 17-12-5 | 46:25 | 21 | 63 |
| 4. | |  | 1. FC Magdeburg | 34 | 17-8-9 | 50:36 | 14 | 59 |
| 5. | |  | VfL Wolfsburg II | 34 | 15-8-11 | 53:35 | 18 | 53 |
| 6. | |  | Hannover 96 II | 34 | 14-7-13 | 48:42 | 6 | 49 |
| 7. |  |  | Chemnitzer FC | 34 | 11-12-11 | 50:42 | 8 | 45 |
| 8. |  |  | VfB Lübeck | 34 | 10-15-9 | 38:35 | 3 | 45 |
| 9. | |  | FC Oberneuland 1948 | 34 | 12-8-14 | 41:41 | 0 | 44 |
| 10. | |  | FC Hansa Rostock II | 34 | 12-7-15 | 53:59 | -6 | 43 |
| 11. | |  | SV Wilhelmshaven | 34 | 12-7-15 | 45:52 | -7 | 43 |
| 12. | |  | Hertha BSC Berlin II | 34 | 11-10-13 | 40:55 | -15 | 43 |
| 13. | |  | Hamburger SV II | 34 | 11-9-14 | 44:43 | 1 | 42 |
| 14. | |  | VFC Plauen | 34 | 10-12-12 | 52:54 | -2 | 42 |
| 15. | |  | Türkiyemspor Berlin | 34 | 12-6-16 | 36:43 | -7 | 42 |
| 16. | |  | Altonaer FC 93 | 34 | 8-7-19 | 40:66 | -26 | 31 |
| 17. | |  | FC Sachsen Leipzig | 34 | 5-9-20 | 21:54 | -33 | 24 |
| 18. | |  | FC Energie Cottbus II | 34 | 5-8-21 | 23:53 | -30 | 23 |