| Sa., 16.05.2009 | VfL Wolfsburg II - Holstein Kiel | 1:1 |
| Sa., 16.05.2009 | VfB Lübeck - FC Oberneuland 1948 | 2:1 |
| Sa., 16.05.2009 | SV Wilhelmshaven - Türkiyemspor Berlin | 2:1 |
| So., 17.05.2009 | Hamburger SV II - Hallescher FC | 1:1 |
| So., 17.05.2009 | Hertha BSC Berlin II - SV Babelsberg 03 | 2:2 |
| So., 17.05.2009 | VFC Plauen - Hannover 96 II | 3:3 |
| Di., 19.05.2009 | FC Hansa Rostock II - FC Energie Cottbus II | 0:2 |
| Mi., 20.05.2009 | FC Sachsen Leipzig - Chemnitzer FC | 0:2 |
| Mi., 20.05.2009 | 1. FC Magdeburg - Altonaer FC 93 | 4:1 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | Holstein Kiel | 31 | 19-9-3 | 50:22 | 28 | 66 |
| 2. | |  | Hallescher FC | 31 | 18-12-1 | 39:17 | 22 | 66 |
| 3. | |  | SV Babelsberg 03 | 31 | 16-11-4 | 44:21 | 23 | 59 |
| 4. |  |  | 1. FC Magdeburg | 31 | 17-6-8 | 46:30 | 16 | 57 |
| 5. |  |  | VfL Wolfsburg II | 31 | 15-7-9 | 52:31 | 21 | 52 |
| 6. | |  | Hannover 96 II | 31 | 12-7-12 | 44:37 | 7 | 43 |
| 7. | |  | SV Wilhelmshaven | 31 | 12-7-12 | 42:46 | -4 | 43 |
| 8. | |  | Hertha BSC Berlin II | 31 | 11-9-11 | 37:48 | -11 | 42 |
| 9. | |  | VfB Lübeck | 31 | 9-14-8 | 34:33 | 1 | 41 |
| 10. | |  | Chemnitzer FC | 31 | 10-10-11 | 47:40 | 7 | 40 |
| 11. | |  | FC Hansa Rostock II | 31 | 11-6-14 | 48:53 | -5 | 39 |
| 12. | |  | FC Oberneuland 1948 | 31 | 10-7-14 | 33:36 | -3 | 37 |
| 13. | |  | VFC Plauen | 31 | 8-11-12 | 47:53 | -6 | 35 |
| 14. | |  | Hamburger SV II | 31 | 8-9-14 | 37:42 | -5 | 33 |
| 15. | |  | Türkiyemspor Berlin | 31 | 9-6-16 | 32:43 | -11 | 33 |
| 16. | |  | Altonaer FC 93 | 31 | 8-5-18 | 36:61 | -25 | 29 |
| 17. | |  | FC Sachsen Leipzig | 31 | 5-9-17 | 20:48 | -28 | 24 |
| 18. | |  | FC Energie Cottbus II | 31 | 5-7-19 | 21:48 | -27 | 22 |