| Fr., 16.10.2009 | FC St. Pauli II - 1. FC Magdeburg | 0:3 |
| Sa., 17.10.2009 | SV Babelsberg 03 - VFC Plauen | 1:0 |
| Sa., 17.10.2009 | Hallescher FC - Türkiyemspor Berlin | 6:0 |
| Sa., 17.10.2009 | VfB Lübeck - SV Wilhelmshaven | 3:0 |
| Sa., 17.10.2009 | Hannover 96 II - FC Hansa Rostock II | 0:2 |
| Sa., 17.10.2009 | VfL Wolfsburg II - FC Oberneuland 1948 | 1:0 |
| So., 18.10.2009 | Hertha BSC Berlin II - ZFC Meuselwitz | 7:3 |
| Di., 20.10.2009 | Goslarer SC 08 - Hamburger SV II | 1:2 |
| Mi., 21.10.2009 | Chemnitzer FC - Tennis Borussia Berlin | 3:0 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | VfL Wolfsburg II | 11 | 7-3-1 | 23:4 | 19 | 24 |
| 2. | |  | SV Babelsberg 03 | 11 | 7-2-2 | 21:9 | 12 | 23 |
| 3. | |  | Hallescher FC | 11 | 6-4-1 | 22:9 | 13 | 22 |
| 4. | |  | Chemnitzer FC | 11 | 6-3-2 | 22:11 | 11 | 21 |
| 5. | |  | VfB Lübeck | 11 | 6-2-3 | 21:15 | 6 | 20 |
| 6. | |  | 1. FC Magdeburg | 11 | 5-4-2 | 24:14 | 10 | 19 |
| 7. |  |  | Hertha BSC Berlin II | 11 | 6-1-4 | 21:16 | 5 | 19 |
| 8. |  |  | Türkiyemspor Berlin | 11 | 5-1-5 | 15:17 | -2 | 16 |
| 9. | |  | Hannover 96 II | 11 | 4-2-5 | 15:13 | 2 | 14 |
| 10. | |  | Hamburger SV II | 11 | 3-5-3 | 12:15 | -3 | 14 |
| 11. | |  | Tennis Borussia Berlin | 11 | 4-2-5 | 9:14 | -5 | 14 |
| 12. | |  | FC St. Pauli II | 11 | 3-4-4 | 11:16 | -5 | 13 |
| 13. | |  | ZFC Meuselwitz | 11 | 2-5-4 | 12:17 | -5 | 11 |
| 14. | |  | SV Wilhelmshaven | 11 | 2-5-4 | 12:18 | -6 | 11 |
| 15. | |  | FC Oberneuland 1948 | 11 | 3-1-7 | 12:25 | -13 | 10 |
| 16. | |  | FC Hansa Rostock II | 11 | 3-1-7 | 14:28 | -14 | 10 |
| 17. | |  | VFC Plauen | 11 | 2-3-6 | 9:17 | -8 | 9 |
| 18. | |  | Goslarer SC 08 | 11 | 1-0-10 | 8:25 | -17 | 3 |