| Fr., 05.03.2010 | SV Babelsberg 03 - ZFC Meuselwitz | 3:0 |
| Sa., 06.03.2010 | Hannover 96 II - Tennis Borussia Berlin | 2:0 |
| Sa., 06.03.2010 | VfB Lübeck - Hertha BSC Berlin II | 1:1 |
| So., 07.03.2010 | Hallescher FC - FC Oberneuland 1948 | 2:1 |
| So., 07.03.2010 | FC Hansa Rostock II - Türkiyemspor Berlin | 3:1 |
| Mi., 14.04.2010 | FC St. Pauli II - Chemnitzer FC | 3:1 |
| Mi., 28.04.2010 | Goslarer SC 08 - VfL Wolfsburg II | 0:4 |
| Mi., 28.04.2010 | VFC Plauen - Hamburger SV II | 2:0 |
| Mi., 28.04.2010 | SV Wilhelmshaven - 1. FC Magdeburg | 1:2 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | SV Babelsberg 03 | 20 | 14-4-2 | 36:13 | 23 | 46 |
| 2. | |  | Chemnitzer FC | 20 | 12-4-4 | 43:20 | 23 | 40 |
| 3. |  |  | Hallescher FC | 19 | 11-6-2 | 34:12 | 22 | 39 |
| 4. |  |  | VfL Wolfsburg II | 19 | 11-5-3 | 31:10 | 21 | 38 |
| 5. | |  | 1. FC Magdeburg | 21 | 9-6-6 | 40:21 | 19 | 33 |
| 6. | |  | Hannover 96 II | 22 | 8-4-10 | 35:29 | 6 | 28 |
| 7. | |  | Hamburger SV II | 17 | 7-6-4 | 24:18 | 6 | 27 |
| 8. | |  | VFC Plauen | 20 | 7-5-8 | 21:25 | -4 | 26 |
| 9. | |  | ZFC Meuselwitz | 19 | 6-7-6 | 22:26 | -4 | 25 |
| 10. | |  | Türkiyemspor Berlin | 21 | 7-4-10 | 29:40 | -11 | 25 |
| 11. | |  | Tennis Borussia Berlin | 21 | 7-4-10 | 19:33 | -14 | 25 |
| 12. | |  | VfB Lübeck | 19 | 7-3-9 | 25:29 | -4 | 24 |
| 13. | |  | FC St. Pauli II | 20 | 6-6-8 | 21:30 | -9 | 24 |
| 14. | |  | FC Hansa Rostock II | 21 | 6-4-11 | 24:43 | -19 | 22 |
| 15. | |  | Hertha BSC Berlin II | 20 | 6-3-11 | 31:39 | -8 | 21 |
| 16. | |  | FC Oberneuland 1948 | 20 | 6-3-11 | 21:36 | -15 | 21 |
| 17. | |  | SV Wilhelmshaven | 18 | 4-6-8 | 23:36 | -13 | 18 |
| 18. | |  | Goslarer SC 08 | 19 | 2-4-13 | 18:37 | -19 | 10 |