| Fr., 19.03.2010 | VfB Lübeck - Chemnitzer FC | 2:0 |
| Sa., 20.03.2010 | Hannover 96 II - 1. FC Magdeburg | 4:1 |
| Sa., 20.03.2010 | Goslarer SC 08 - Tennis Borussia Berlin | 0:0 |
| Sa., 20.03.2010 | FC St. Pauli II - FC Oberneuland 1948 | 4:4 |
| Sa., 20.03.2010 | Hertha BSC Berlin II - VfL Wolfsburg II | 1:3 |
| So., 21.03.2010 | Hallescher FC - ZFC Meuselwitz | 2:0 |
| So., 21.03.2010 | VFC Plauen - FC Hansa Rostock II | 0:2 |
| So., 21.03.2010 | SV Wilhelmshaven - Türkiyemspor Berlin | 1:1 |
| So., 21.03.2010 | SV Babelsberg 03 - Hamburger SV II | 0:0 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | SV Babelsberg 03 | 23 | 15-6-2 | 37:13 | 24 | 51 |
| 2. |  |  | Hallescher FC | 22 | 12-8-2 | 37:13 | 24 | 44 |
| 3. | |  | VfL Wolfsburg II | 21 | 13-5-3 | 35:11 | 24 | 44 |
| 4. |  |  | Chemnitzer FC | 23 | 12-6-5 | 45:24 | 21 | 42 |
| 5. | |  | 1. FC Magdeburg | 24 | 9-7-8 | 42:27 | 15 | 34 |
| 6. | |  | Hannover 96 II | 23 | 9-4-10 | 39:30 | 9 | 31 |
| 7. | |  | VfB Lübeck | 22 | 9-3-10 | 31:32 | -1 | 30 |
| 8. | |  | VFC Plauen | 23 | 8-6-9 | 27:31 | -4 | 30 |
| 9. | |  | Hamburger SV II | 20 | 7-8-5 | 26:21 | 5 | 29 |
| 10. | |  | ZFC Meuselwitz | 22 | 7-8-7 | 25:30 | -5 | 29 |
| 11. | |  | FC Hansa Rostock II | 24 | 8-5-11 | 29:45 | -16 | 29 |
| 12. | |  | Tennis Borussia Berlin | 24 | 7-7-10 | 22:36 | -14 | 28 |
| 13. | |  | Türkiyemspor Berlin | 23 | 7-5-11 | 32:45 | -13 | 26 |
| 14. | |  | FC St. Pauli II | 21 | 6-7-8 | 25:34 | -9 | 25 |
| 15. | |  | FC Oberneuland 1948 | 22 | 6-5-11 | 25:40 | -15 | 23 |
| 16. | |  | Hertha BSC Berlin II | 22 | 6-4-12 | 34:44 | -10 | 22 |
| 17. | |  | SV Wilhelmshaven | 20 | 4-7-9 | 24:38 | -14 | 19 |
| 18. | |  | Goslarer SC 08 | 21 | 2-5-14 | 19:40 | -21 | 11 |