| Di., 01.09.2009 | FC Hansa Rostock II - Hamburger SV II | 1:2 |
| Di., 01.09.2009 | Hallescher FC - VfL Wolfsburg II | 2:2 |
| Di., 01.09.2009 | Goslarer SC 08 - Chemnitzer FC | 0:2 |
| Di., 01.09.2009 | VfB Lübeck - Türkiyemspor Berlin | 1:0 |
| Mi., 02.09.2009 | SV Wilhelmshaven - ZFC Meuselwitz | 0:0 |
| Mi., 02.09.2009 | VFC Plauen - 1. FC Magdeburg | 1:4 |
| Mi., 02.09.2009 | Hannover 96 II - FC Oberneuland 1948 | 5:0 |
| Mi., 02.09.2009 | SV Babelsberg 03 - Tennis Borussia Berlin | 0:1 |
| Mi., 02.09.2009 | FC St. Pauli II - Hertha BSC Berlin II | 3:1 |
| Sp. | S-U-N | Tore | Diff. | P | ||||
|---|---|---|---|---|---|---|---|---|
| 1. |  |  | VfL Wolfsburg II | 5 | 3-2-0 | 10:2 | 8 | 11 |
| 2. |  |  | VfB Lübeck | 5 | 3-2-0 | 8:3 | 5 | 11 |
| 3. | |  | Tennis Borussia Berlin | 5 | 3-1-1 | 7:4 | 3 | 10 |
| 4. | |  | Hannover 96 II | 4 | 3-0-1 | 9:2 | 7 | 9 |
| 5. |  |  | Hallescher FC | 5 | 2-3-0 | 10:6 | 4 | 9 |
| 6. | |  | Türkiyemspor Berlin | 5 | 3-0-2 | 6:5 | 1 | 9 |
| 7. | |  | Chemnitzer FC | 5 | 2-2-1 | 7:6 | 1 | 8 |
| 8. | |  | FC Hansa Rostock II | 5 | 2-1-2 | 9:10 | -1 | 7 |
| 9. | |  | 1. FC Magdeburg | 5 | 1-3-1 | 7:5 | 2 | 6 |
| 10. | |  | ZFC Meuselwitz | 5 | 1-3-1 | 5:4 | 1 | 6 |
| 11. | |  | Hamburger SV II | 5 | 1-3-1 | 4:4 | 0 | 6 |
| 12. | |  | SV Babelsberg 03 | 5 | 1-2-2 | 3:4 | -1 | 5 |
| 13. | |  | FC St. Pauli II | 4 | 1-1-2 | 7:8 | -1 | 4 |
| 14. | |  | Hertha BSC Berlin II | 5 | 1-1-3 | 5:9 | -4 | 4 |
| 15. | |  | FC Oberneuland 1948 | 5 | 1-1-3 | 8:14 | -6 | 4 |
| 16. | |  | SV Wilhelmshaven | 5 | 0-3-2 | 5:8 | -3 | 3 |
| 17. | |  | Goslarer SC 08 | 5 | 1-0-4 | 5:12 | -7 | 3 |
| 18. | |  | VFC Plauen | 5 | 0-2-3 | 3:12 | -9 | 2 |